State Key Laboratory of Microbial Technology, National Glycoengineering Research Center, Shandong University, Qingdao, 266237, Shandong, People's Republic of China.
Curr Microbiol. 2020 Jan;77(1):49-54. doi: 10.1007/s00284-019-01803-8. Epub 2019 Nov 7.
CpcA is a conserved transcriptional activator for the cross-pathway control of amino acid biosynthetic genes in filamentous fungi. Previous studies of this regulator mainly revealed its function under amino acid starvation condition, where amino acid biosynthetic inhibitors were added in the culture. In this study, the biological function of CpcA in Penicillium oxalicum was investigated under different cultivation conditions. Disruption of cpcA led to decreased cell growth either in the presence or absence of histidine biosynthetic inhibitor, and the phenotype could be rescued by the addition of exogenous amino acid sources. In addition, CpcA was required for the rapid production of cellulase when cells were cultured on cellulose. Transcript abundance measurement showed that a set of amino acid biosynthetic genes as well as two major cellulase genes were significantly down-regulated in cpcA deletion mutant relative to wild type. Taken together, the results revealed the biological role of CpcA in supporting normal growth and extracellular enzyme production of P. oxalicum under amino acid non-starvation condition.
CpcA 是一种保守的转录激活因子，可用于丝状真菌中氨基酸生物合成基因的交叉途径控制。该调节剂的先前研究主要揭示了其在氨基酸饥饿条件下的功能，即在培养物中添加氨基酸生物合成抑制剂的情况下。在这项研究中，研究了 CpcA 在草酸青霉中的生物学功能在不同的培养条件下。cpcA 的破坏导致细胞生长无论是在组氨酸生物合成抑制剂的存在或不存在的情况下，并且表型可以通过添加外源氨基酸源来挽救。此外，当细胞在纤维素上培养时，CpcA 需要快速产生纤维素酶。转录丰度测量显示，一组氨基酸生物合成基因以及两种主要的纤维素酶基因在 cpcA 缺失突变体中相对于野生型显著下调。总之，这些结果揭示了 CpcA 在氨基酸非饥饿条件下支持草酸青霉正常生长和细胞外酶产生的生物学作用。
