Wang Ru-Huan, Zhou Ru, Ding Yang, Zhou Zhen-Xing
Shenzhen Salubris Pharmaceutical Co., Ltd, Shenzhen 518102.
Department of Clinical Laboratory, Beijing Ditan Hospital Affiliated to Capital Medical University, Beijing 100015.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2019 Jul 28;35(4):359-362. doi: 10.12047/j.cjap.5797.2019.076.
To investigate the effects of berberine on learning and memory ability in vascular cognitive impairment rats.
Sixty-eight Wistar rats were randomly divided into control group (n=10), sham operated group (n=10) and the modeling group of vascular cognitive impairment rat (n=48), then the rats in modeling group were randomly divided into four groups (n=10): vehicle group, berberine low dose group (20 mg/kg), medium dose group (40 mg/kg) and high dose group (60 mg/kg). Bilateral common carotid arteries were occluded in rats to establish vascular cognitive impairment (VCI) model. Different doses of berberine were intraperitoneally injected into the treatment group and normal saline was intraperitoneally injected into the other groups once a day for a total of 34 days. After 28 days of administration, Morris water maze was used to test the learning and memory ability of rats. After the water maze experiment, the levels of superoxide dismutase (SOD) activity, glutathione (GSH), malondialdehyde (MDA), tumor necrosis factor alpha(TNF-α), interleukin-1 beta (IL-1β), 5-hydroxytryptamine (5-HT) and monoamine oxidase (MAO) in the forebrain cortex were detected.
Compared to sham group, the escape latency in VCI group was significantly extended (P＜0.01) and the times of passing through the platform were decreased remarkably (P＜0.01). The levels of SOD, GSH and 5-HT in the hippocampus or anterior cortex were decreased significantly (P＜0.01), while the contents of MDA, TNF-α, IL-1β and MAO were increased remarkably (P＜0.01). Compared with VCI group, the escape latency in berberine-treated groups was shortened significantly (P＜0.01, P＜0.05) and the times of passing through the platform were increased remarkably (P＜0.01, P＜0.05), the levels of SOD, GSH and 5-HT were increased significantly (P＜0.01), while the contents of TNF-α, IL-1β and MAO were decreased remarkably (P＜0.01).
Berberine could significantly improve the spatial learning and memory abilities of rats with vascular cognitive impairment. The mechanism may be related to the effects of berberine on the hippocampal antioxidant stress, anti-inflammatory response and the monoamine neurotransmitter system in the forebrain cortex. Berberine 60 mg/kg dose group had better effect.
探讨黄连素对血管性认知障碍大鼠学习记忆能力的影响。
将68只Wistar大鼠随机分为对照组（n = 10）、假手术组（n = 10）和血管性认知障碍大鼠模型组（n = 48），然后将模型组大鼠随机分为四组（n = 10）：溶剂组、黄连素低剂量组（20 mg/kg）、中剂量组（40 mg/kg）和高剂量组（60 mg/kg）。通过结扎大鼠双侧颈总动脉建立血管性认知障碍（VCI）模型。治疗组腹腔注射不同剂量的黄连素，其他组腹腔注射生理盐水，每日1次，共34天。给药28天后，采用Morris水迷宫检测大鼠的学习记忆能力。水迷宫实验结束后，检测大鼠前脑皮质中超氧化物歧化酶（SOD）活性、谷胱甘肽（GSH）、丙二醛（MDA）、肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-1β）、5-羟色胺（5-HT）和单胺氧化酶（MAO）的水平。
与假手术组相比，VCI组大鼠逃避潜伏期显著延长（P＜0.01），穿越平台次数显著减少（P＜0.01）。海马或前额叶皮质中SOD、GSH和5-HT水平显著降低（P＜0.01），而MDA、TNF-α、IL-1β和MAO含量显著增加（P＜0.01）。与VCI组相比，黄连素治疗组大鼠逃避潜伏期显著缩短（P＜0.01，P＜0.05），穿越平台次数显著增加（P＜0.01，P＜0.05），SOD、GSH和5-HT水平显著升高（P＜0.01），而TNF-α、IL-1β和MAO含量显著降低（P＜0.01）。
黄连素可显著改善血管性认知障碍大鼠的空间学习记忆能力。其机制可能与黄连素对海马抗氧化应激、抗炎反应及前额叶皮质单胺能神经递质系统的作用有关。黄连素60 mg/kg剂量组效果更佳。
