Department of Dermatology, Institute of Dermatology, the First Affiliated Hospital, Anhui Medical University, Hefei 230032, PR China.
The Key Laboratory of Dermatology (Anhui Medical University), Ministry of Education, Hefei 230032, PR China.
Epigenomics. 2019 Nov;11(14):1613-1625. doi: 10.2217/epi-2018-0225. Epub 2019 Nov 8.
To understand whether the anatomical location of origin plays a role in shaping the DNA methylation (DNAm) landscape of psoriatic skins. A number of 108 psoriatic and 57 control skin samples were grouped based on their anatomical locations. Two group t-tests were used to identify those differentially methylated sites and regions. Target region methylation loci were validated by bisulfate conversion sequencing. The correlations of DNAm with pathological features, DNAm and gene expression were also interrogated. Our analysis revealed 315 location-specific differentially methylated sites for back, 291 for the extremities and 801 for abdomen. Moreover, we observed that the extremity-specific loci cg21942490 located on is associated with hyperkeratosis. We further observed that and are differential methylation regions. Our study shown evidence of anatomical location-dependent DNAm pattern in psoriasis skins, and thus provided new insights into the pathogenesis of this disease.
为了探究银屑病皮肤中 DNA 甲基化（DNAm）的形成是否与解剖学起源位置有关，我们根据解剖位置将 108 例银屑病皮肤和 57 例对照皮肤样本分为不同的组。采用双样本 t 检验来识别差异甲基化的位点和区域。通过亚硫酸氢盐测序来验证靶向区域甲基化基因座。我们还分析了 DNAm 与病理特征、DNAm 与基因表达之间的相关性。我们的分析显示，背部有 315 个位置特异性差异甲基化位点，四肢有 291 个，腹部有 801 个。此外，我们观察到位于 上的特定位点 cg21942490 与过度角化有关。我们进一步观察到 和 是差异甲基化区域。本研究证明了银屑病皮肤中存在与解剖位置相关的 DNAm 模式，为该疾病的发病机制提供了新的见解。
