College of Food Science and Engineering, Northwest A&F University, Yangling, China.
Department of Clinical Nutrition, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.
Foodborne Pathog Dis. 2020 Apr;17(4):243-252. doi: 10.1089/fpd.2019.2729. Epub 2019 Nov 8.
Necrotizing enterocolitis (NEC) is a serious inflammatory intestinal disorder with a high mortality rate, which occurs most commonly in newborn infants. , a common contaminant in infant formula, is associated with NEC. However, its role in NEC pathogenesis is unknown, and there are still no effective treatments for NEC. Currently, natural bioactive products have been investigated for their beneficial effects in preventing microbial infection. In this study, a neonatal mouse intestinal inflammation model was used to examine the protective effects of citral (a natural bioactive product) on -induced intestinal inflammation and damages. It was shown that citral reduced the number of cells in ileal tissues, and mice treated with citral had a significantly higher body weight than -infected mice. Citral treatment also ameliorated serious ileal tissue damages, including epithelial sloughing, villous rupture, and enterocyte apoptosis. infection upregulated the messenger RNA transcription levels of several inflammation-associated genes, increased production of IL-6 and TNF-α, and activated the NF-κB and MAPK signaling pathways in ileal tissues. Citral treatment mitigated these inflammatory responses. The apoptotic index and activities of caspase 3, 8, and 9 increased in murine ileum after infection, but citral inhibited both enterocyte apoptosis and activations of these caspase. These findings suggest that citral has protective effects on -induced intestinal inflammation in newborn mice, and it may play a future role in the management of associated infections and diseases.
坏死性小肠结肠炎（NEC）是一种严重的炎症性肠道疾病，死亡率较高，常见于新生儿。，婴儿配方奶粉中的常见污染物，与 NEC 有关。然而，其在 NEC 发病机制中的作用尚不清楚，目前仍没有有效的 NEC 治疗方法。目前，天然生物活性产物已被研究用于预防微生物感染的有益作用。在本研究中，使用新生小鼠肠道炎症模型来研究柠檬醛（一种天然生物活性产物）对诱导的肠道炎症和损伤的保护作用。结果表明，柠檬醛减少了回肠组织中的细胞数量，并用柠檬醛处理的小鼠的体重比感染的小鼠显著增加。柠檬醛处理还改善了严重的回肠组织损伤，包括上皮脱落、绒毛破裂和肠细胞凋亡。感染上调了几个炎症相关基因的信使 RNA 转录水平，增加了 IL-6 和 TNF-α 的产生，并激活了回肠组织中的 NF-κB 和 MAPK 信号通路。柠檬醛处理减轻了这些炎症反应。感染后，小鼠回肠中的细胞凋亡指数和 caspase 3、8 和 9 的活性增加，但柠檬醛抑制了肠细胞凋亡和这些 caspase 的激活。这些发现表明，柠檬醛对新生小鼠诱导的肠道炎症具有保护作用，它可能在相关感染和疾病的治疗中发挥作用。
