Tao Xiaoying, Cheng Lili, Li Yijie, Ci Hongfei, Xu Jing, Wu Shiwu, Tao Yisheng
Department of Pathology, First Affiliated Hospital of Bengbu Medical College.
Department of Pathology, Bengbu Medical College, Bengbu, China.
Medicine (Baltimore). 2019 Nov;98(45):e17799. doi: 10.1097/MD.0000000000017799.
Alpha-B crystallin (CRYAB), as a small heat shock protein, has been found to be highly expressed in various human cancers and significantly associated with the unfavorable prognosis of these tumor. Nevertheless, the clinical significance of CRYAB in gastric cancer (GC) angiogenesis remains to be elucidated. In this study, we evaluated the expression of CRYAB and CD34 in GC tissues and corresponding normal gastric specimens to explore whether high level CRYAB is related with the angiogenesis and the poor prognosis in GC.In this study, the expression of CRYAB and CD34 were detected in GC tissues and corresponding normal gastric tissues by immunohistochemical (IHC) technique. Furthermore, the relationship of CRYAB with CD34-evaluated microvessel density (MVD) and poor prognosis was also investigated.CRYAB expression level was significantly higher in GC tissue than in normal gastric mucosa tissue, and clearly mean higher MVD was observed in tumor tissues compared with non-cancerous tissues. Besides, higher MVD value was observed in positive CRYAB expression group than in negative CRYAB expression group. Statistical analysis showed that CRYAB and MVD are associated with clinicopathological features including lymph node metastasis (LNM), tumor differentiation, invasion depth, and TNM stages. Kaplan-Meier method and multivariate survival analysis indicated that high expression of CRYAB, MVD, invasion depth, TNM stages, and tumor differentiation, as well as LNM significantly correlate with poor prognosis of GC patients.High expression of CRYAB may contribute to angiogenesis, invasion and metastasis of GC. These results indicated that CRYAB was expected to be a promising molecular marker for poor prognosis and potential therapeutic target in patients with GC.
α-B晶状体蛋白（CRYAB）作为一种小分子热休克蛋白，已发现在多种人类癌症中高度表达，并与这些肿瘤的不良预后显著相关。然而，CRYAB在胃癌（GC）血管生成中的临床意义仍有待阐明。在本研究中，我们评估了CRYAB和CD34在GC组织及相应正常胃标本中的表达，以探讨CRYAB高表达是否与GC的血管生成及不良预后相关。在本研究中，采用免疫组织化学（IHC）技术检测GC组织及相应正常胃组织中CRYAB和CD34的表达。此外，还研究了CRYAB与CD34评估的微血管密度（MVD）及不良预后的关系。CRYAB在GC组织中的表达水平显著高于正常胃黏膜组织，且肿瘤组织中的MVD明显高于非癌组织。此外，CRYAB阳性表达组的MVD值高于阴性表达组。统计分析表明，CRYAB和MVD与包括淋巴结转移（LNM）、肿瘤分化、浸润深度和TNM分期在内的临床病理特征相关。Kaplan-Meier法和多因素生存分析表明，CRYAB高表达、MVD、浸润深度、TNM分期、肿瘤分化以及LNM均与GC患者的不良预后显著相关。CRYAB高表达可能促进GC的血管生成、侵袭和转移。这些结果表明，CRYAB有望成为GC患者不良预后的有前景的分子标志物和潜在治疗靶点。
