Li Sheng, Chai Xiao Ning, Zuo Chuan Yi, Lv Peng, Tang Yong, Tan Hui Juan, Liu Li Zhou, Yin Hai Yan, Yu Shu Guang
Chengdu University of Traditional Chinese Medicine, Chengdu.
Southwest Medical University, Luzhou, China.
Medicine (Baltimore). 2019 Nov;98(45):e17843. doi: 10.1097/MD.0000000000017843.
Acupuncture therapy is frequently used to treat Knee Osteoarthritis (KOA) in clinic, and usually used local acupoints near the diseased knees as therapeutic targets. Some local acupoints appeared sensitization phenomenon which was called sensitized acupoints, which were regarded as important therapeutic targets to get better therapeutic effect on clinic. Therefore, it is necessary to explore the biological basis of acupoint sensitization. Meanwhile, there is a lack of an analysis of the metabolism for sensitized acupoints in KOA patients. Considering that acupuncture effect could be multi-targeted, omics (such as metabolomics) may be a useful method to reveal the relationship between sensitized acupoints and clinical efficacy of acupuncture.
This study is a parallel design trial. Thirty KOA patients and 30 healthy volunteers will be recruited in this study. Mechanical pain threshold will be measured by Electron Von frey in order to confirm the highest sensitized acupoints. Then collect tissue fluid from the highest sensitized acupoints by micro dialysis technical, then apply electro-acupuncture method on the highest sensitized acupoints to treat KOA patients, after 20 sessions treatments, measure and collect again. Liquid chromatography-tandem mass spectrometry method will be used to analyze the metabonomics of dialysate.
This study will provide a high-quality evidence to reveal the local molecular mechanism of acupuncture sensitized acupoints for patient with KOA.
This study will provide up-date evidence of whether acupuncture sensitized acupoints have local molecular mechanism for KOA.
NCT03599180 (24 Jul. 2018).
针刺疗法在临床上常用于治疗膝骨关节炎（KOA），通常将患病膝关节附近的局部穴位作为治疗靶点。一些局部穴位出现了敏化现象，即所谓的敏化腧穴，在临床上被视为获得更好治疗效果的重要治疗靶点。因此，有必要探索穴位敏化的生物学基础。同时，目前缺乏对KOA患者敏化穴位的代谢分析。鉴于针刺效应可能是多靶点的，组学（如代谢组学）可能是揭示敏化穴位与针刺临床疗效之间关系的有用方法。
本研究为平行设计试验。本研究将招募30例KOA患者和30名健康志愿者。采用电子von frey测定机械性疼痛阈值，以确定最高敏化穴位。然后通过微透析技术从最高敏化穴位采集组织液，接着对最高敏化穴位采用电针方法治疗KOA患者，20次治疗后，再次进行测量和采集。采用液相色谱-串联质谱法分析透析液的代谢组学。
本研究将为揭示针刺敏化穴位治疗KOA患者的局部分子机制提供高质量证据。
本研究将为针刺敏化穴位治疗KOA是否具有局部分子机制提供最新证据。
NCT03599180（2018年7月24日）。
