Gil-Loyzaga P, Brownell W E
INSERM-U.254, C.H.R., Hopital de St. Charles, Montpellier, France.
Hear Res. 1988 Jul 15;34(2):149-55. doi: 10.1016/0378-5955(88)90102-5.
The fluorescein labelled lectins FITC-WGA and FITC-HPA were used to identify specific carbohydrates in cochlear hair cells. Wheat germ agglutinin (WGA) bound with the cell coat of both inner and outer hair cells (IHC and OHC) suggesting the presence of either N-acetyl-D-glucosamine or sialic acid. In contrast, glycoconjugates with terminal N-acetyl-D-galactosamine residues that bind with Helix pomatia agglutinin (HPA), were demonstrated inside the plasma membrane of outer hair cells. WGA and HPA lectin binding implies the presence of anionic glycoconjugates that furnish added negative charge on the membranes to which they are fixed. The presence of sialic acid or N-acetyl-D-glucosamine on the extracellular surface of cochlear hair cell plasma membrane is consistent with the normal distribution of these glycoconjugates in the cell coat. The presence of the membrane associated oligosaccharide N-acetyl-D-galactosamine within the outer hair cell is inconsistent with the distribution of glycoproteins in internal membrane systems of other cell types.
用荧光素标记的凝集素FITC-WGA和FITC-HPA来鉴定耳蜗毛细胞中的特定碳水化合物。小麦胚凝集素（WGA）与内、外毛细胞（IHC和OHC）的细胞被膜结合，表明存在N-乙酰-D-葡萄糖胺或唾液酸。相比之下，与蜗牛凝集素（HPA）结合的带有末端N-乙酰-D-半乳糖胺残基的糖缀合物，在外毛细胞的质膜内被证实。WGA和HPA凝集素结合意味着存在阴离子糖缀合物，它们在其固定的膜上提供额外的负电荷。耳蜗毛细胞质膜细胞外表面存在唾液酸或N-乙酰-D-葡萄糖胺，与这些糖缀合物在细胞被膜中的正常分布一致。外毛细胞内存在与膜相关的寡糖N-乙酰-D-半乳糖胺，与其他细胞类型内膜系统中糖蛋白的分布不一致。
