Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, China; College of Life Science, Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Neijiang Normal University, Neijiang, Sichuan, 641100, China.
China Institute of Water Resources and Hydropower Research, Beijing, 100044, China.
Environ Toxicol Pharmacol. 2020 Jan;73:103278. doi: 10.1016/j.etap.2019.103278. Epub 2019 Oct 15.
A 56-day trial was conducted to elucidate the bioconcentration and depuration of Cd in the liver and muscle of rare minnow (Gobiocypris rarus) and determine the effect of dietary mulberry leaf supplementation on depuration. Juvenile rare minnow were exposed to environmentally relevant doses of Cd (1 and 10 μg/L) for 28 days of uptake and then allowed 28 days of depuration. The bioaccumulation factors of the treated rare minnow in the liver and muscle were calculated to be between 4.13-4.675 and 1.76-1.94, respectively. This results suggested that Cd had high potential for bioconcentration in rare minnow. To investigate the effect of dietary mulberry leaf supplementation on depuration, the remaining fish of each group were allowed to depurate with different ratios (0%, 10%, and 30% dry weight) of dietary mulberry leaf supplementation for an additional 28 days. Fish weights did not differ significantly (p > 0.05) between the control and mulberry leaf treated groups. Mulberry leaf powder did not significantly affect Cd depuration in the 10 μg/L group or in the muscle of the 1 μg/L group, but caused a significant decrease in Cd content in the liver of the 1 μg/L group (p < 0.05). This work was the first to model the bioconcentration of Cd in rare minnow and showed that mulberry leaf supplementation decreased Cd residues in the liver of the 1μg/L group. Such a finding may promote the development of new approaches to mitigate the potential hazards of heavy metals to human health.
进行了一项为期 56 天的试验，以阐明 Cd 在稀有鲫（Gobiocypris rarus）肝脏和肌肉中的生物浓缩和净化作用，并确定桑叶补充剂对净化的影响。将幼年稀有鲫暴露于环境相关剂量的 Cd（1 和 10μg/L）中 28 天进行吸收，然后允许 28 天进行净化。处理过的稀有鲫肝脏和肌肉中的生物累积因子分别计算为 4.13-4.675 和 1.76-1.94。这表明 Cd 具有在稀有鲫中生物浓缩的高潜力。为了研究桑叶补充剂对净化的影响，每组剩余的鱼在净化过程中分别添加不同比例（0%、10%和 30%干重）的桑叶补充剂，再进行 28 天。对照组和桑叶处理组的鱼体重无显著差异（p>0.05）。桑叶粉对 10μg/L 组或 1μg/L 组肌肉中的 Cd 净化没有显著影响，但导致 1μg/L 组肝脏中的 Cd 含量显著降低（p<0.05）。这项工作首次对 Cd 在稀有鲫中的生物浓缩进行了建模，并表明桑叶补充剂降低了 1μg/L 组肝脏中的 Cd 残留。这一发现可能会促进开发新方法来减轻重金属对人类健康的潜在危害。
