隔日 5-氟尿嘧啶对头颈部癌细胞抗癌作用的研究。
Study of the Anti-cancer Effect of Alternate-day 5-Fluorouracil in Head and Neck Cancer Cells.
机构信息
Department of Otolaryngology, Head and Neck Surgery, Kyorin University School of Medicine, Tokyo, Japan.
Department of Head and Neck Surgery, Otolaryngology, Saitama Medical University International Medical Center, Saitama, Japan
出版信息
Anticancer Res. 2019 Nov;39(11):6041-6047. doi: 10.21873/anticanres.13810.
BACKGROUND/AIM: We have previously reported that alternate-day S-1 had comparable effects and milder adverse events than the respective consecutive-day regimen in head and neck cancer (HNC) patients. The aim of this study was to investigate the anticancer effects of both regimens and underlying mechanisms in vitro.
MATERIALS AND METHODS
Two head and neck squamous cell carcinoma (HNSCC) cell lines were treated with 5-FU given on an alternate-day or consecutive-day schedule. The relative inhibition (RI) of tumor growth was calculated. Cell cycle distributions and cyclin expression following 5-FU treatment were analyzed.
RESULTS
The RI of both regimens was almost identical. The percentage of cells in S phase was significantly increased in the alternate-day group compared to the consecutive-day group (p<0.001).
CONCLUSION
The cytotoxic effect of alternate-day was equivalent to that of consecutive-day. S-phase arrest was more prominently observed with the alternate-day regimen, which may help maintain 5-FU sensitivity in head and neck cancer cells.
背景/目的：我们之前的研究报告表明，在头颈部癌症（HNC）患者中，隔日 S-1 方案的疗效与连续日方案相当，且不良反应更轻微。本研究旨在体外研究两种方案的抗癌作用及其潜在机制。
材料和方法
两种头颈部鳞状细胞癌（HNSCC）细胞系分别接受隔日或连续日 5-FU 治疗。计算肿瘤生长的相对抑制率（RI）。分析 5-FU 处理后细胞周期分布和细胞周期蛋白的表达。
结果
两种方案的 RI 几乎相同。与连续日组相比，隔日组 S 期细胞的百分比显著增加（p<0.001）。
结论
隔日的细胞毒性作用与连续日相当。隔日方案更明显地观察到 S 期阻滞，这可能有助于维持头颈部癌细胞对 5-FU 的敏感性。
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