磷酸二酯酶 2A 在人骨肉瘤细胞增殖和迁移中的作用。
Role of Phosphodiesterase2A in Proliferation and Migration of Human Osteosarcoma Cells.
机构信息
Department of Oral and Maxillofacial Surgery, Department of Clinical Sciences, Medical Life Science, Mie University, Graduate School of Medicine, Mie, Japan
Department of Oral and Maxillofacial Surgery, Department of Clinical Sciences, Medical Life Science, Mie University, Graduate School of Medicine, Mie, Japan.
出版信息
Anticancer Res. 2019 Nov;39(11):6057-6062. doi: 10.21873/anticanres.13812.
BACKGROUND/AIM: The prognosis of patients with osteosarcoma is poor; therefore, new treatment strategies are urgently needed. Phosphodiesterase 2 (PDE2) is one of the 11 families (PDE1-PDE11) of the phosphodiesterase superfamily that regulates the intracellular concentrations and effects of cAMP and cGMP. This in vitro study was performed to investigate the role of PDE2 in human oral osteosarcoma HOSM-1 cells.
MATERIALS AND METHODS
PDE2 expression was measured by a cAMP-PDE assay and real-time-PCR. The effects of the PDE2-specific inhibitors, erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), 8-bromo-cAMP, and 8-bromo-cGMP on cell proliferation and migration were assessed.
RESULTS
PDE2 activity and PDE2A mRNA expression were detected in HOSM-1 cells. Cell proliferation was inhibited by EHNA and 8-bromo-cAMP but not by 8-bromo-cGMP. Cell migration was stimulated by EHNA and 8-bromo-cGMP, but it was inhibited by 8-bromo-cAMP.
CONCLUSION
Cell proliferation is regulated by PDE2-cAMP signaling and cell migration is regulated by PDE2-cGMP signaling in HOSM-1 cells.
背景/目的：骨肉瘤患者的预后较差；因此，迫切需要新的治疗策略。磷酸二酯酶 2（PDE2）是磷酸二酯酶超家族的 11 个家族（PDE1-PDE11）之一，可调节细胞内 cAMP 和 cGMP 的浓度和作用。本体外研究旨在探讨 PDE2 在人口腔骨肉瘤 HOSM-1 细胞中的作用。
材料和方法
通过 cAMP-PDE 测定和实时 PCR 测量 PDE2 表达。评估 PDE2 特异性抑制剂，赤式-9-(2-羟基-3-壬基)腺嘌呤（EHNA）、8-溴-cAMP 和 8-溴-cGMP 对细胞增殖和迁移的影响。
结果
在 HOSM-1 细胞中检测到 PDE2 活性和 PDE2A mRNA 表达。EHNA 和 8-溴-cAMP 抑制细胞增殖，但 8-溴-cGMP 不抑制细胞增殖。EHNA 和 8-溴-cGMP 刺激细胞迁移，但 8-溴-cAMP 抑制细胞迁移。
结论
在 HOSM-1 细胞中，细胞增殖受 PDE2-cAMP 信号调节，细胞迁移受 PDE2-cGMP 信号调节。
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