抑制肌肽对人结直肠癌细胞黏附和渗出的作用。

Suppression of Carnosine on Adhesion and Extravasation of Human Colorectal Cancer Cells.

机构信息

Department of Food and Nutrition, Providence University, Taichung, Taiwan, R.O.C.

Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, R.O.C.

出版信息

Anticancer Res. 2019 Nov;39(11):6135-6144. doi: 10.21873/anticanres.13821.

DOI:10.21873/anticanres.13821

PMID:31704841

Abstract

AIM

To investigate the effect of carnosine, an active compound of dietary beef, fish and chicken, on the regulation of cell adhesion and extravasation during metastasis.

MATERIALS AND METHODS

Cell adhesion and extravasation abilities, and related regulating molecular mechanisms were analyzed in human colorectal cancer cells (HCT-116) and human umbilical vein cells (EA.hy926).

RESULTS

Carnosine reduced the ability of HCT-116 cells to adhere to EA.hy926 cells. The expression levels of integrin-β1 in HCT-116 cells, as well as of intercellular adhesion molecule-1 and E-selectin in EA.hy926 cells, were reduced after carnosine treatment. After EA.hy926 cells were treated with carnosine, phosphorylation of vascular endothelia-cadherin (VE-cadherin), protein levels of Ras homologous (RHO) and RHO-associated coiled-coil containing protein kinase, and levels of reactive oxygen species were reduced. After treating EA.hy926 cells with carnosine, phosphorylation of inhibitor of kappa B (IκB) and DNA binding activity of nuclear factor-κB (NF-κB) were reduced.

CONCLUSION

Carnosine inhibits metastatic cell adhesion and extravasation by suppressing NF-κB signaling activation.

摘要

目的

研究膳食牛肉、鱼肉和鸡肉中的活性化合物——肌肽对转移过程中细胞黏附和渗出的调节作用。

材料与方法

分析了人结直肠癌细胞（HCT-116）和人脐静脉细胞（EA.hy926）的细胞黏附和渗出能力及其相关调节分子机制。

结果

肌肽降低了 HCT-116 细胞与 EA.hy926 细胞的黏附能力。肌肽处理后，HCT-116 细胞中整合素-β1的表达水平以及 EA.hy926 细胞中细胞间黏附分子-1 和 E-选择素的表达水平均降低。在用肌肽处理 EA.hy926 细胞后，血管内皮钙黏蛋白（VE-cadherin）的磷酸化、Ras 同源物（RHO）和 RHO 相关卷曲螺旋蛋白激酶的蛋白水平以及活性氧水平降低。在用肌肽处理 EA.hy926 细胞后，IκB 的磷酸化和核因子-κB（NF-κB）的 DNA 结合活性降低。