嵌合抗原受体T细胞（CAR-T细胞）的免疫表型分析

Immunophenotypic Analysis of CAR-T Cells.

作者信息

de Azevedo Júlia Teixeira Cottas, Mizukami Amanda, Moço Pablo Diego, Malmegrim Kelen Cristina Ribeiro

机构信息

Center for Cell-based Therapy, Regional Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.

出版信息

Methods Mol Biol. 2020;2086:195-201. doi: 10.1007/978-1-0716-0146-4_14.

DOI:10.1007/978-1-0716-0146-4_14

PMID:31707677

Abstract

CAR-T cell immunotherapy is a promising therapeutic modality for cancer patients. The success of CAR-T cell therapy has been associated with the phenotype, activation and functional profiling of infused CAR-T cells. Therefore, immunophenotypic characterization of CAR-T cells during bioprocess is crucial for cell quality control and ultimately for improved antitumor efficacy. In this chapter, we propose a flow cytometry panel to characterize the immunophenotype of the CAR-T subsets.

摘要

嵌合抗原受体（CAR）T细胞免疫疗法是一种对癌症患者很有前景的治疗方式。CAR-T细胞疗法的成功与输注的CAR-T细胞的表型、活化及功能分析相关。因此，在生物过程中对CAR-T细胞进行免疫表型特征分析对于细胞质量控制以及最终提高抗肿瘤疗效至关重要。在本章中，我们提出了一个流式细胞术检测方案来鉴定CAR-T细胞亚群的免疫表型。