使用氟化和新型洗脱添加剂在反相柱上保留酸性和碱性分析物，用于液相色谱-串联质谱法。
Retention of acidic and basic analytes in reversed phase column using fluorinated and novel eluent additives for liquid chromatography-tandem mass spectrometry.
机构信息
University of Tartu, Institute of Chemistry, 14a Ravila Street, 50411 Tartu, Estonia; Analytical Services International, St George's University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom.
University of Tartu, Institute of Chemistry, 14a Ravila Street, 50411 Tartu, Estonia.
出版信息
J Chromatogr A. 2020 Feb 22;1613:460667. doi: 10.1016/j.chroma.2019.460667. Epub 2019 Nov 1.
This research focuses on retention mechanisms in a LC column with C18 stationary phase when novel eluent additives (HFIP, HFTB and TFE as well as NFTB and perfluoropinacol) are used. The retention factors between novel eluent additives and conventional ones like ammonium acetate and ammonium bicarbonate at different eluent pH values were compared. A simple set of drug-like molecules, widely spread over different logP values, containing protonated and deprotonated acids and bases was selected for this investigation. HFIP, HFTB, NFTB and PP demonstrated strong influence on basic polar analytes in basic medium. These additives drastically increased retention. A decrease in retention was observed for acidic analytes when novel eluent additives were used. Additionally, for the first time, the absolute pH (pH) scale was used for expressing the mobile phase pH.
本研究关注的是在使用新型流动相添加剂（HFIP、HFTB 和 TFE 以及 NFTB 和全氟双酚 A）时，C18 固定相的 LC 柱保留机制。比较了新型流动相添加剂与传统添加剂（如乙酸铵和碳酸氢铵）之间在不同流动相 pH 值下的保留因子。选择了一组简单的类药分子，它们广泛分布在不同的 logP 值范围内，包含质子化和去质子化的酸和碱，用于这项研究。HFIP、HFTB、NFTB 和 PP 对碱性介质中的碱性极性分析物有很强的影响。这些添加剂大大增加了保留。当使用新型流动相添加剂时，酸性分析物的保留时间会降低。此外，这是首次在表达流动相 pH 值时使用绝对 pH（pH）标度。
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