Desai Rupali K, Fernandez Maria Salvador, Wijffels Rene H, Eppink Michel H M
Bioprocess Engineering, AlgaePARC, Wageningen University, Wageningen, Netherlands.
Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
Front Bioeng Biotechnol. 2019 Oct 25;7:284. doi: 10.3389/fbioe.2019.00284. eCollection 2019.
Microalgae are a promising source for proteins, lipids, and carbohydrates for the food/feed and biofuel industry. To make microalgae production economically feasible, it is necessary to optimally use all produced compounds keeping full functionality. Therefore, biorefining of microalgae is the key to lower the cost of algal products using mild and effective processing techniques. In this article, we have tested the feasibility of aqueous solutions of imidazolium and phosponium ionic liquids to selectively milk the hydrophobic lipids from biomass out of intact cells and recover after cell disruption the hydrophilic fraction containing proteins and carbohydrates. The results showed that the ionic liquid tributylmethylphosphonium methylsulfate (TBP SO; Cyphos 108) is able to permeabilize fresh intact cells of for extracting 68% of total lipids out of the cells, whereas, after cell disruption, 80% of total proteins, and 77% of total carbohydrates could be obtained in aqueous buffers. This concept kept the recovered proteins in their native form without interacting with the ionic liquids that will denature the proteins. Selective biorefinery of different components from microalgae using ionic liquid TBP SO explains the novelty of this concept.
微藻是食品/饲料和生物燃料行业中蛋白质、脂质和碳水化合物的一个有前景的来源。为了使微藻生产在经济上可行，有必要以最佳方式利用所有产生的化合物并保持其全部功能。因此，微藻的生物精炼是使用温和且有效的加工技术降低藻类产品成本的关键。在本文中，我们测试了咪唑鎓和鏻离子液体水溶液从完整细胞的生物质中选择性提取疏水性脂质，并在细胞破碎后回收包含蛋白质和碳水化合物的亲水性部分的可行性。结果表明，离子液体硫酸三丁基甲基鏻（TBP SO；Cyphos 108）能够使新鲜的完整细胞透化，以从细胞中提取68%的总脂质，而在细胞破碎后，在水性缓冲液中可获得80%的总蛋白质和77%的总碳水化合物。这一概念使回收的蛋白质保持其天然形式，而不与会使蛋白质变性的离子液体相互作用。使用离子液体TBP SO对微藻中不同成分进行选择性生物精炼解释了这一概念的新颖性。
