Department of Chemistry, Faculty of Science, Gebze Technical University, Kocaeli, Turkey.
Department of Biological Sciences, Middle East Technical University, Ankara, Turkey.
Luminescence. 2020 Mar;35(2):168-177. doi: 10.1002/bio.3719. Epub 2019 Nov 10.
BODIPY is an important fluorophores due to its enhanced photophysical and chemical properties including outstanding thermal/photochemical stability, intense absorption/emission profiles, high photoluminescence quantum yield, and small Stokes' shifts. In addition to BODIPY, indole and its derivatives have recently gained attention because of their structural properties and particularly biological importance, therefore these molecules have been widely used in sensing and biosensing applications. Here, we focus on recent studies that reported the incorporation of indole-based BODIPY molecules as reporter molecules in sensing systems. We highlight the rationale for developing such systems and evaluate detection limits of the developed sensing platforms. Furthermore, we also review the application of indole-based BODIPY molecules in bioimaging studies. This article includes the evaluation of indole-based BODIPYs from synthesis to characterization and a comparison of the advantages and disadvantages of developed reporter systems, making it instructive for researchers in various disciplines for the design and development of similar systems.
BODIPY 是一种重要的荧光团，由于其增强的光物理和化学性质，包括出色的热/光化学稳定性、强烈的吸收/发射谱、高光致发光量子产率和小的斯托克斯位移。除了 BODIPY，吲哚及其衍生物因其结构特性和特别的生物学重要性而受到关注，因此这些分子已被广泛应用于传感和生物传感应用中。在这里，我们重点关注最近报道的将基于吲哚的 BODIPY 分子作为报告分子纳入传感系统的研究。我们强调了开发此类系统的基本原理，并评估了所开发传感平台的检测限。此外，我们还回顾了基于吲哚的 BODIPY 分子在生物成像研究中的应用。本文包括从合成到表征对基于吲哚的 BODIPY 的评估，以及对开发的报告系统的优缺点的比较，这对各个学科的研究人员在设计和开发类似系统时具有指导意义。
