Institute of Molecular Functional Materials (Areas of Excellence Scheme, University Grants Committee (Hong Kong)) and Department of Chemistry , The University of Hong Kong , Pokfulam Road , Hong Kong , People's Republic of China.
Department of Chemistry , City University of Hong Kong , Tat Chee Avenue , Kowloon , Hong Kong , People's Republic of China.
J Am Chem Soc. 2019 Nov 20;141(46):18570-18577. doi: 10.1021/jacs.9b09515. Epub 2019 Nov 11.
Amyloid fibrillation has been acknowledged as a hallmark of a number of neurodegenerative ailments such as Alzheimer's disease. Accordingly, efficient detection of amyloid fibrillation will allow for great advances in the field of biomedical applications as well as in achieving early medical diagnosis. In this work, a luminescence assay for the sensitive and specific detection of amyloid fibrillation was developed by using platinum(II) complexes as sensing platforms. Supramolecular self-assembly of platinum(II) complexes was induced upon addition of amyloid, leading to alterations in the spectroscopic and luminescence properties of the complexes. As compared to fluorescent dyes, luminescent platinum(II) complexes exhibit attractive large Stokes shifts, phosphorescence lifetimes in the microsecond to submicrosecond regime, and low-energy red emission after aggregation, which are advantageous to biological imaging. At the same time, the platinum(II) complex adopted herein was found to have high photostability, high selectivity and specificity, and low cytotoxicity. The proposed design is the very first approach to detect amyloid fibrillation through the supramolecular self-assembly of luminescent platinum(II) complexes.
淀粉样纤维已被公认为许多神经退行性疾病（如阿尔茨海默病）的标志性特征。因此，有效地检测淀粉样纤维将极大地促进生物医学应用领域的发展，并有助于实现早期医学诊断。在这项工作中，我们使用铂(II)配合物作为传感平台，开发了一种用于灵敏和特异地检测淀粉样纤维的荧光分析方法。淀粉样纤维的加入诱导了铂(II)配合物的超分子自组装，导致配合物的光谱和荧光性质发生变化。与荧光染料相比，发光铂(II)配合物具有吸引人的大斯托克斯位移、微秒到亚微秒范围内的磷光寿命以及聚集后的低能量红光发射，这有利于生物成像。同时，本文采用的铂(II)配合物具有高的光稳定性、高的选择性和特异性以及低的细胞毒性。所提出的设计是通过发光铂(II)配合物的超分子自组装来检测淀粉样纤维的首例方法。
