Department of Ophthalmology, Taipei Tzu Chi Hospital, The Buddhist Tzu Chi Medical Foundation, Taipei, Taiwan; College of Medicine, Tzu Chi University, Hualien, Taiwan; Department of Ophthalmology, National Taiwan University Hospital, Medical College, National Taiwan University, Taipei, Taiwan.
Department of Ophthalmology, National Taiwan University Hospital, Medical College, National Taiwan University, Taipei, Taiwan.
Cont Lens Anterior Eye. 2020 Aug;43(4):338-344. doi: 10.1016/j.clae.2019.10.137. Epub 2019 Nov 9.
To compare the sensitivity of two genotypes of P. aeruginosa to various disinfectant solutions and analyze the attached bacteria on worn cosmetic contact lenses (cosCLs).
In this prospective study, healthy volunteers wore etafilcon (brown), nelfilcon (gray), or hilafilcon (black) cosCLs and microbial adhesion analysis was performed. A rub-off test determined pigment dislodgement. Disinfectant sensitivity to Optifree Replenish (Alcon), Optifree Pure Moist (Alcon), Renu Fresh (Bausch & Lomb), and AoSept Plus (Ciba Vision) was tested at various disinfection times and compared between various genotypes and Type III secretion (T3S) system mutants.
Of the 1152 cosCLs collected, 364 were culture positive (32%). The highest rate of culture-positive lens was hilafilcon (chi square, P = 0.0001). Hilafilcon also had a significantly greater number of isolates than etafilcon (P < 0.0001). Hilafilcon was the only lens to fail the rub-off test. Cytotoxic strains were significant more resistant to Renu Fresh than were invasive strains, even at 100% of recommended disinfection time (P = 0.0005). Of the tested disinfectants, Renu Fresh was significantly less effective in killing both genotypes of P. aeruginosa compared to AoSept Plus at all time points (25%, 50%, 75%, and 100% recommended disinfection time, P = 0.0001, 0.0001, 0.0005, and 0.0005, respectively). When the T3S system was dysfunctional, mutant strains were all susceptible to disinfectants (P = 0.0001 for both invasive and cytotoxic strains).
Pseudomonas species is commonly found on cosCLs of asymptomatic individuals. Wearers of cosCLs that dislodge pigments may be predisposed to microbial contamination. Cytotoxic strains are more resistant to disinfectant solutions, especially to Renu Fresh. P. aeruginosa disinfectant resistance requires a functional T3S system.
比较两种铜绿假单胞菌基因型对各种消毒剂的敏感性，并分析磨损的美容隐形眼镜（cosCL）上的附着细菌。
在这项前瞻性研究中，健康志愿者佩戴依视康（棕色）、海昌（灰色）或酷柏（黑色）的 cosCL，并进行微生物黏附分析。摩擦试验确定颜料脱落情况。用 Optifree Replenish（爱尔康）、Optifree Pure Moist（爱尔康）、Renu Fresh（博士伦）和 AoSept Plus（CIBA Vision）消毒剂检测不同消毒时间下对各种基因型和 III 型分泌系统（T3S）突变体的消毒敏感性，并进行比较。
共收集到 1152 片 cosCL，其中 364 片培养阳性（32%）。培养阳性镜片中，酷柏的比例最高（卡方检验，P=0.0001）。与依视康相比，酷柏的分离株数量也明显更多（P<0.0001）。只有酷柏的镜片未能通过摩擦试验。细胞毒性株对 Renu Fresh 的耐药性明显高于侵袭性株，即使在推荐消毒时间的 100%（P=0.0005）时也是如此。在测试的消毒剂中，与 AoSept Plus 相比，Renu Fresh 在所有时间点对两种铜绿假单胞菌的杀灭效果均明显较差（推荐消毒时间的 25%、50%、75%和 100%，P=0.0001、0.0001、0.0005 和 0.0005）。当 T3S 系统功能失调时，突变株对消毒剂均敏感（侵袭性株和细胞毒性株均 P=0.0001）。
无症状个体的 cosCL 上通常会发现铜绿假单胞菌。脱落颜料的 cosCL 佩戴者可能容易受到微生物污染。细胞毒性株对消毒剂的耐药性更强，尤其是对 Renu Fresh。铜绿假单胞菌消毒剂耐药性需要功能性 T3S 系统。
