Kader Farzeen, Ghai Meenu, Olaniran Ademola O
Discipline of Genetics, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, Republic of South Africa.
Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban, Republic of South Africa.
Int J Legal Med. 2020 Jan;134(1):1-20. doi: 10.1007/s00414-019-02181-3. Epub 2019 Nov 12.
Body fluid identification in crime scene investigations aids in reconstruction of crime scenes. Several studies have identified and reported differentially methylated sites (DMSs) and regions (DMRs) which differ between forensically relevant tissues (tDMRs) and body fluids. Diverse factors affect methylation patterns such as the environment, diets, lifestyle, disease, ethnicity, genetic variation, amongst others. Thus, it is important to analyse the stability of markers employed for forensic identification. Furthermore, even though epigenetic modifications are described as stable and heritable, epigenetic inheritance of potential markers for body fluid identification needs to be assessed in the long term. Here, we discuss the current status of reported DNA methylation-based markers and their verification studies. Such thorough investigation is crucial to develop a stable panel of DNA methylation-based markers for accurate body fluid identification.
犯罪现场调查中的体液鉴定有助于重建犯罪现场。多项研究已经鉴定并报告了法医相关组织（tDMRs）和体液之间存在差异的甲基化位点（DMSs）和区域（DMRs）。多种因素会影响甲基化模式，如环境、饮食、生活方式、疾病、种族、基因变异等。因此，分析用于法医鉴定的标记物的稳定性很重要。此外，尽管表观遗传修饰被描述为稳定且可遗传的，但用于体液鉴定的潜在标记物的表观遗传遗传需要长期评估。在此，我们讨论已报道的基于DNA甲基化的标记物的现状及其验证研究。如此全面的调查对于开发一组稳定的基于DNA甲基化的标记物以进行准确的体液鉴定至关重要。
