Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Nigeria.
Andrologia. 2020 Feb;52(1):e13454. doi: 10.1111/and.13454. Epub 2019 Nov 13.
Tramadol is widely abused in Nigeria and has been reported to cause fertility decline via testicular oxidative stress. This study investigated the effect of vitamin E, an antioxidant on some reproductive parameters in male Wistar rats administered tramadol. Twenty male Wistar rats (180-200 g) were randomly assigned into four groups (n = 5) thus: Control (0.2 ml vehicle: olive oil), tramadol-treated (20 mg/kg of tramadol), vitamin E-treated (100 mg/kg of vitamin E) and tramadol + vitamin E-treated (received tramadol and vitamin E) groups. Drugs were administered orally and daily for 28 days. Sperm count, Johnsen's score, germinal epithelial height and serum testosterone, follicle-stimulating hormone (FSH) and luteinising hormone (LH) concentrations were significantly (p < .05) decreased in tramadol-treated and tramadol + vitamin E compared with control and vitamin E-treated groups. Sperm motility, morphology, viability, seminiferous tubular diameter, Leydig cell count, Sertoli cell count and malondialdehyde, superoxide dismutase, glutathione peroxidase and catalase concentrations were not significantly different among the groups. Histology of testis and epididymis in all groups showed no toxicity but decreased sperm population in tramadol-treated and tramadol + vitamin E-treated groups. Tramadol did not cause testicular oxidative stress but impaired testicular function by suppressing testosterone, FSH and LH secretion. Vitamin E administration could not attenuate this impairment in testicular function.
曲马多在尼日利亚被广泛滥用，并已被报道通过睾丸氧化应激导致生育能力下降。本研究调查了抗氧化剂维生素 E 对给予曲马多的雄性 Wistar 大鼠一些生殖参数的影响。将 20 只雄性 Wistar 大鼠（180-200g）随机分为四组（n=5）：对照组（0.2ml 载体：橄榄油）、曲马多处理组（20mg/kg 曲马多）、维生素 E 处理组（100mg/kg 维生素 E）和曲马多+维生素 E 处理组（给予曲马多和维生素 E）。药物每天口服给予，持续 28 天。曲马多处理组和曲马多+维生素 E 处理组的精子计数、约翰森评分、生殖上皮高度以及血清睾酮、卵泡刺激素（FSH）和黄体生成素（LH）浓度显著（p<.05）降低，与对照组和维生素 E 处理组相比。精子运动、形态、活力、曲细精管直径、Leydig 细胞计数、Sertoli 细胞计数以及丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶浓度在各组之间无显著差异。各组睾丸和附睾组织学检查未见毒性，但曲马多处理组和曲马多+维生素 E 处理组的精子数量减少。曲马多未引起睾丸氧化应激，但通过抑制睾酮、FSH 和 LH 分泌损害睾丸功能。维生素 E 给药不能减轻这种睾丸功能损害。
