Sawada H
Department of Fine Morphology, University of Tokyo, Japan.
J Microsc. 1988 Jun;150(Pt 3):219-23. doi: 10.1111/j.1365-2818.1988.tb04640.x.
In order to investigate the ultrastructural three-dimensional relationship between extracellular matrices (ECM) and the plasma membranes of cultured cells, a freeze-etch replica method was devised. Bovine corneal endothelial cells were cultured on a Collodion film which covered a hole punched in a plastic coverslip, and were quickly frozen with a slammer with their basal surface facing a liquid nitrogen-cooled copper block. The cells were placed upside-down in a Balzers freeze-fracture machine and freeze-etched, and then platinum-carbon replicas were obtained. The structure of the ECM-plasma membrane interface was observed successfully and so this technique provides a new approach for investigating the ECM-plasma membrane (matrix-receptor) relationship.
为了研究细胞外基质（ECM）与培养细胞的质膜之间的超微结构三维关系，设计了一种冷冻蚀刻复型方法。将牛角膜内皮细胞培养在覆盖有塑料盖玻片上打孔的火棉胶膜上，使其基底表面面对液氮冷却的铜块，用 slammer 快速冷冻。将细胞倒置放入 Balzers 冷冻断裂机中进行冷冻蚀刻，然后获得铂 - 碳复型。成功观察到了 ECM - 质膜界面的结构，因此该技术为研究 ECM - 质膜（基质 - 受体）关系提供了一种新方法。
