Molecular Microbial Ecology Group (gEMM-IEA), Institute of Aquatic Ecology, Faculty of Sciences, University of Girona, Campus Montilivi, Maria Aurèlia Capmany 40, E-17003, Girona, Catalonia, Spain.
LEQUIA. Institute of Environment. University of Girona, Campus Montilivi, Maria Aurèlia Capmany 69, E-17003, Girona, Catalonia, Spain.
Chemosphere. 2020 Feb;240:124908. doi: 10.1016/j.chemosphere.2019.124908. Epub 2019 Sep 18.
Organosilicon compounds are the most undesirable compounds for the energy recovery of biogas. These compounds are still resistant to biodegradation when biotechnologies are considered for biogas purification. Herein we isolated 52 bacterial species from anaerobic batch enrichment cultures (BEC) saturated with D4 and from an anaerobic lab-scale biotrickling filter (BTF) fed with a gas flow containing D4 as unique carbon source. Among those Methylibium sp. and Pseudomonas aeruginosa showed the highest capacity to remove D4 (53.04% ± 0.03 and 24.42% ± 0.02, respectively). Contrarily, co-culture evaluation treatment for the biodegradation of siloxanes together with volatile organic compounds removed a lower concentration of D4 compared to toluene and limonene, which were completely removed. Remarkably, the siloxane D5 proved to be more biodegradable than D4. Substrates removal values achieved by Methylibium sp. suggested that this bacterial isolate could be used in biological removal technologies of siloxanes.
有机硅化合物是沼气能源回收最不理想的化合物。当考虑生物技术对沼气进行净化时，这些化合物仍然具有抗生物降解性。在此，我们从 D4 饱和的厌氧批量富集培养物 (BEC) 和以 D4 作为唯一碳源的厌氧实验室规模生物滴滤器 (BTF) 中分离出 52 种细菌。其中，甲基杆菌和铜绿假单胞菌显示出最高的 D4 去除能力（分别为 53.04%±0.03 和 24.42%±0.02）。相反，与甲苯和柠檬烯相比，硅氧烷和挥发性有机化合物的共培养评价处理去除了较低浓度的 D4，而甲苯和柠檬烯则被完全去除。值得注意的是，硅氧烷 D5 比 D4 更具生物降解性。甲基杆菌达到的基质去除值表明，这种细菌分离株可用于硅氧烷的生物去除技术。
