Spada Sheila, Yamazaki Takahiro, Vanpouille-Box Claire
Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, United States.
Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, United States.
Methods Enzymol. 2019;629:17-33. doi: 10.1016/bs.mie.2019.07.042. Epub 2019 Aug 9.
Cytosolic DNA sensing is emerging to be a critical component of the antitumor immune response by jumpstarting innate immune responses subsequent to the stimulation of the cGAS and STING pathway. Investigating the accumulation of DNA species in the cytosol is therefore an essential readout for promising anticancer strategies. In this chapter, we present different techniques that can be utilized to detect and quantify cytosolic DNA accumulation.
胞质DNA传感正逐渐成为抗肿瘤免疫反应的关键组成部分，它通过启动cGAS和STING通路刺激后的固有免疫反应来实现。因此，研究胞质中DNA种类的积累是有前景的抗癌策略的一项重要指标。在本章中，我们介绍了可用于检测和定量胞质DNA积累的不同技术。
