1Department of Molecular Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, NSW 2109 Australia.
2School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052 Australia.
NPJ Biofilms Microbiomes. 2019 Nov 6;5(1):34. doi: 10.1038/s41522-019-0108-3. eCollection 2019.
Infections caused by are increasingly antibiotic resistant, generating a significant public health problem. Like many bacteria, adopts a biofilm lifestyle that enhances its antibiotic resistance and environmental resilience. Biofilms represent the predominant mode of microbial life, but research into antibiotic resistance has mainly focused on planktonic cells. We investigated the dynamics of biofilms in the presence of antibiotics. A 3-day exposure of biofilms to sub-inhibitory concentrations of antibiotics had a profound effect, increasing biofilm formation and antibiotic resistance in the majority of biofilm dispersal isolates. Cells dispersing from biofilms were genome sequenced to identify mutations accumulating in their genomes, and network analysis linked these mutations to their phenotypes. Transcriptomics of biofilms confirmed the network analysis results, revealing novel gene functions of relevance to both resistance and biofilm formation. This approach is a rapid and objective tool for investigating resistance dynamics of biofilms.
由 引起的感染对抗生素的耐药性越来越强，这是一个严重的公共卫生问题。与许多细菌一样， 采用生物膜生活方式，从而增强其对抗生素的耐药性和对环境的适应能力。生物膜代表了微生物的主要生存方式，但对抗生素耐药性的研究主要集中在浮游细胞上。我们研究了抗生素存在下 生物膜的动态变化。将 生物膜暴露于亚抑制浓度的抗生素中 3 天，对大多数生物膜分散分离株的生物膜形成和抗生素耐药性产生了深远的影响。从生物膜中分散出来的细胞进行了基因组测序，以鉴定其基因组中积累的突变，并通过网络分析将这些突变与它们的表型联系起来。生物膜的转录组学证实了网络分析的结果，揭示了与耐药性和生物膜形成相关的新基因功能。这种方法是研究生物膜耐药性动态的快速而客观的工具。
