The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, China.
School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, China.
Microsc Res Tech. 2020 Mar;83(3):268-275. doi: 10.1002/jemt.23410. Epub 2019 Nov 14.
In this study, a ternary nanocomposite consisting of gold nanoparticles (AuNPs), hydroxyapatite (HAP) nanowires, and reduced graphene oxide (rGO) is synthesized by a simple one-step hydrothermal method, which is used to modify glassy carbon electrode (GCE) for detecting uric acid. The nanocomposite is characterized through various methods such as scanning electron microscopy, transmission electron microscopy, and X-ray diffraction. Electrochemical measurements of the modified GCE are performed in a conventional three-electrode system. Experimental results show that the obtained HAP nanowire and rGO are mixed homogeneously, and the AuNPs are deposited into this matrix. The GCE modified by the nanocomposites have superior electrocatalytic activities for uric acid. The peak current intensities of UAO (uricase)/HAP-rGO/AuNPs sensing system linearly increase as the uric acid concentration increases substantially in a range of 1.95 × 10 to 6.0 × 10 M (R = .9943), with a detection limit of 3.9 × 10 M (S/N = 3) and analytical sensitivity of 13.86 mA/M. The biosensor performs well in determining uric acid concentration in human urine samples.
在这项研究中，通过一种简单的一步水热法合成了由金纳米粒子（AuNPs）、羟基磷灰石（HAP）纳米线和还原氧化石墨烯（rGO）组成的三元纳米复合材料，用于修饰玻碳电极（GCE）以检测尿酸。通过扫描电子显微镜、透射电子显微镜和 X 射线衍射等多种方法对纳米复合材料进行了表征。在传统的三电极系统中对修饰后的 GCE 进行了电化学测量。实验结果表明，所获得的 HAP 纳米线和 rGO 均匀混合，并且 AuNPs 沉积在该基质中。纳米复合材料修饰的 GCE 对尿酸具有优异的电催化活性。在尿酸酶（UAO）/HAP-rGO/AuNPs 传感系统中，随着尿酸浓度在 1.95×10 到 6.0×10 范围内显著增加，峰值电流强度线性增加 （R =.9943），检测限为 3.9×10 （S/N = 3），分析灵敏度为 13.86 mA/M。该生物传感器在测定人尿样中的尿酸浓度方面表现良好。
