Faculty of Sciences and Techniques Errachidia, Moulay Ismail University of Meknes, BP 509, Boutalamine, 52000. Errachidia, Morocco.
Division of Preventive Oncology, National Center for Tumor Diseases, Im Neuenheimer Feld 460, German Cancer Research Center (DKFZ), Im Neuenheimer Feld 581, Heidelberg, Germany.
Endocr Metab Immune Disord Drug Targets. 2020;20(7):1059-1072. doi: 10.2174/1871530319666191115114023.
This study aimed to evaluate the effect of the aqueous extract of Anvillea radiate (A. radiata) aerial parts (AEAR) on arterial blood pressure in normotensive and hypertensive rats.
The effect of the acute and sub-chronic administration of AEAR on the following blood pressure parameters: systolic blood pressure (SBP), mean blood pressure (MBP), diastolic blood pressure (DBP), and heart rate (HR) was evaluated in normotensive and L-NAME induced hypertensive rats. In the second experiment, the vasorelaxant effect of AEAR was assessed in isolated aortic rings from rats with functional endothelium pre-contracted with epinephrine (EP) or KCl, and six antagonists/ inhibitors were used to explore the mechanisms of action involved in the vasorelaxant effect. In order to determine the phytochemical contents of Anvillea radiata, HPLC-ESI-MS analysis was conducted.
Daily oral administration of AEAR (100 mg/kg) provoked a significant decrease in SBP, MBP, and DBP without affecting HR in hypertensive rats. In addition, AEAR (0.08-0.64 mg/ml) revealed a vasorelaxant effect in thoracic aortic rings pre-contracted by EP (10 μM) or KCl (80 mM). This effect was reduced in the presence of Nifedipine, L-Name or Methylene blue. The polyphenolic compounds of AEAR were determined.
This study revealed that AEAR possesses a potent antihypertensive activity and its vasorelaxant activity seems to be mediated through Ca2+ channels, direct nitric oxide (NO), and NO/cGMP pathways. Chlorogenic acid and caffeic acid identified in A. radiata could be at least partially responsible for the antihypertensive activity of this extract.
本研究旨在评估艾纳香（Anvillea radiate）地上部分水提物（AEAR）对正常血压和高血压大鼠动脉血压的影响。
在正常血压和 L-NAME 诱导的高血压大鼠中，评估 AEAR 急性和亚慢性给药对以下血压参数的影响：收缩压（SBP）、平均血压（MBP）、舒张压（DBP）和心率（HR）。在第二项实验中，使用六类拮抗剂/抑制剂来评估 AEAR 在预先用肾上腺素（EP）或 KCl 收缩的大鼠离体主动脉环中的血管舒张作用，并探讨涉及血管舒张作用的作用机制。为了确定艾纳香的植物化学成分，进行了 HPLC-ESI-MS 分析。
每天口服 AEAR（100mg/kg）可显著降低高血压大鼠的 SBP、MBP 和 DBP，而不影响 HR。此外，AEAR（0.08-0.64mg/ml）对 EP（10μM）或 KCl（80mM）预收缩的胸主动脉环具有血管舒张作用。在存在硝苯地平、L-Name 或亚甲蓝的情况下，这种作用会降低。确定了 AEAR 的多酚化合物。
本研究表明，AEAR 具有强大的降压活性，其血管舒张活性似乎通过 Ca2+通道、直接一氧化氮（NO）和 NO/cGMP 途径介导。在艾纳香中鉴定出的绿原酸和咖啡酸可能至少部分负责该提取物的降压活性。
