串联质谱法在酰基肉碱定量分析中的应用

The Use of DMS-MS for the Quantitative Analysis of Acylcarnitines.

作者信息

Vera Nicholas B, Clasquin Michelle, Coy Stephen L, Vouros Paul

机构信息

Cambridge Laboratories, Pfizer Global Research and Development, Pfizer Inc., Cambridge, MA, USA.

Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA.

出版信息

Methods Mol Biol. 2020;2084:95-101. doi: 10.1007/978-1-0716-0030-6_5.

DOI:10.1007/978-1-0716-0030-6_5

PMID:31729655

Abstract

Differential mobility spectrometry (DMS) is capable of separating molecules based on their size and shape. When coupled to mass spectrometry (MS), DMS reduces chemical background and enhances signal-to-noise (S/N) ratio. Flow injection analysis (FIA) is a technique used to introduce samples into the source of the DMS-MS platform. Here we describe the application of FIA-DMS-MS/MS for the analysis of urinary acylcarnitine species. More than 20 acylcarnitine species can be detected and quantified during a single FIA-DMS-MS/MS acquisition.

摘要

差分离子迁移谱（DMS）能够根据分子的大小和形状对其进行分离。当与质谱（MS）联用时，DMS可降低化学背景并提高信噪比（S/N）。流动注射分析（FIA）是一种用于将样品引入DMS-MS平台源的技术。在此，我们描述了FIA-DMS-MS/MS在分析尿酰基肉碱种类中的应用。在单次FIA-DMS-MS/MS采集过程中可检测和定量20多种酰基肉碱种类。