Department of Kinesiology, University of Wisconsin - Madison, Madison, WI, USA; Program in Occupational Therapy, University of Wisconsin - Madison, Madison, WI, USA; Department of Biomedical Engineering, University of Wisconsin - Madison, Madison, WI, USA.
Wisconsin National Primate Research Center, University of Wisconsin - Madison, Madison, WI, USA.
J Neurosci Methods. 2020 Jan 15;330:108517. doi: 10.1016/j.jneumeth.2019.108517. Epub 2019 Nov 12.
Objective gait evaluation in humans is used as a predictive disability outcome measure as well as an indicator for intervention effectiveness. Parallel methods of gait analysis in nonhuman primate models are essential for clinical translation. The goal of this study was to first assess whether marmosets' gait data could be reliably collected in a Noldus CatWalk XT10.6 and second, establish a testing protocol to assess gait and the intraindividual variability during repeated testing.
The CatWalk, originally developed for rodents, was modified and used to assess gait in eight adult common marmoset monkeys across multiple days and trials. Data was first analyzed to identify valid runs. Repeated measures ANOVA was completed for the following gait measures: mean base of support, average stride length, average swing time, and average stance time.
Raters had a high level of concurrence of usable data across all trials with successful trials including four consecutive hindfoot footfalls, during a continuous, uninterrupted segment of walking. A significant main effect of time (p < 0.000) but not rater (p = 0.98) was present with significant interactions for time by subject (p < 0.000), but not rater per subject (p = 0.538), time (p = 0.186), or three-way interaction (p = 0.297).
COMPARISON WITH EXISTING METHOD(S): Gait has been assessed using force-plate and video data. The CatWalk allowed reproducible, automated and translational locomotor data to be collected at multiple time points with detailed analyses that identified a diagonal gait pattern.
The CatWalk system, similar to those used in humans, can be effectively used to quantify spatiotemporal characteristics of gait in the common marmoset.
在人类中，客观的步态评估既可用作预测残疾结果的测量指标，也可用作干预效果的指标。在非人类灵长类动物模型中，平行的步态分析方法对于临床转化至关重要。本研究的目的首先是评估是否可以在 Noldus CatWalk XT10.6 中可靠地收集狨猴的步态数据，其次是建立一个测试方案来评估步态和重复测试中的个体内变异性。
最初为啮齿动物开发的 CatWalk 经过修改后，用于评估 8 只成年普通狨猴在多个天和试验中的步态。首先对数据进行分析以识别有效运行。对以下步态测量值进行重复测量方差分析：平均基底支持、平均步长、平均摆动时间和平均站立时间。
在所有试验中，评分者对所有试验的可用数据都有很高的一致性，成功的试验包括连续四个后脚足印，在连续、不间断的行走过程中。时间的主效应（p<0.000）且评分者（p=0.98）显著，存在时间与受试者的显著交互作用（p<0.000），但评分者与受试者的交互作用不显著（p=0.538），时间的交互作用（p=0.186）或三因素交互作用（p=0.297）不显著。
步态已经使用力板和视频数据进行了评估。CatWalk 允许在多个时间点重复收集可重现、自动化和转化的运动数据，并进行详细分析，以确定对角线步态模式。
与人类中使用的系统类似，CatWalk 系统可有效用于量化普通狨猴步态的时空特征。
