Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov 410049, Russia.
Vavilov Saratov State Agrarian University, Saratov 410012, Russia.
Int J Biol Macromol. 2020 Jul 1;154:1375-1381. doi: 10.1016/j.ijbiomac.2019.11.017. Epub 2019 Nov 13.
Lipopolysaccharide (LPS) of Ochrobactrum cytisi IPA7.2, a bacterium isolated from the roots of Solanum tuberosum L., was extracted from dry bacterial cells and chemically characterized. The O-specific polysaccharide was obtained by mild acid hydrolysis of the LPS and studied by sugar analysis and H and C NMR spectroscopy, including H,H COSY, H,H TOCSY, H,H ROESY, H,C HSQC, and H,C HMBC experiments. The polysaccharide was linear and consisted of trisaccharide repeating units of the following structure: A putative O-antigen gene cluster of O. cytisi IPA7.2 was identified and found to be consistent with the O-specific polysaccharide structure. The LPS of Ochrobactrum cytisi IPA7.2 promoted the growth of potato microplants in vitro.
从分离自马铃薯根际的欧文氏菌（Ochrobactrum cytisi）IPA7.2 中提取脂多糖（LPS），并对其进行了化学表征。从 LPS 中经温和酸水解得到 O-特异性多糖，通过糖分析和 1H 和 13C NMR 光谱（包括 H,H COSY、H,H TOCSY、H,H ROESY、H,C HSQC 和 H,C HMBC 实验）对其进行了研究。该多糖为线性结构，由以下结构的三糖重复单元组成：鉴定到欧文氏菌（Ochrobactrum cytisi）IPA7.2 的 O-抗原基因簇，其与 O-特异性多糖结构一致。Ochrobactrum cytisi IPA7.2 的 LPS 可促进马铃薯微体植物在体外生长。
