Qasemi Research Center, Al-Qasemi Academic College, P.O. Box 124, Baqa El-Gharbia 30100, Israel.
The Mina and Everard Goodman Faculty of Life Science, Bar Ilan University, Ramat Gan 5299, Israel.
Molecules. 2019 Nov 15;24(22):4139. doi: 10.3390/molecules24224139.
Naturally derived drugs and plant-based products are attractive commodities that are being explored for cancer treatment. This in vitro study aimed to investigate the role of (50% ethanol: 50% water) extract (HTE) treatment on apoptosis, cell cycle modulation, and cell cycle arrest in human colon cancer cell line (HCT-116). HTE induced cell death via an apoptotic process, as assayed by an Annexin V-Cy3 assay. Exposing HCT-116 cells to 0.064, 0.125, 0.25, and 0.5 mg/mL of HTE for 24 h led to 50 ± 9%, 71.6 ± 8%, 85 ± 5%, and 96 ± 1.5% apoptotic cells, respectively. HCT-116 cells treated with 0.25 and 0.5 mg/mL HTE for 3 h resulted in 38.9 ± 1.5% and 57.2 ± 3% cleavage of caspase-3-specific substrate, respectively. RT-PCR analysis revealed that the HTE extract had no effect on mRNA levels of Apaf-1 and NOXA. Moreover, the addition of 0.125 mg/mL and 0.25 mg/mL HTE for 24 h was clearly shown to attenuate the cell cycle progression machinery in HCT-116 cells. GC/MS analysis of the extract identified 21 phytochemicals that are known as apoptosis inducers and cell cycle arrest agents. All the compounds detected are novel in . These results suggest that HTE-induced apoptosis of human colon cells is mediated primarily through the caspase-dependent pathway. Thus, HTE appears to be a potent therapeutic agent for colon cancer treatment.
天然药物和植物产品是具有吸引力的商品，正在被探索用于癌症治疗。本体外研究旨在研究（50%乙醇：50%水）提取物（HTE）处理在人结肠癌细胞系（HCT-116）中的细胞凋亡、细胞周期调节和细胞周期阻滞中的作用。HTE 通过细胞凋亡测定法（Annexin V-Cy3 测定法）诱导细胞死亡。将 HCT-116 细胞暴露于 0.064、0.125、0.25 和 0.5 mg/mL 的 HTE 24 h，分别导致 50±9%、71.6±8%、85±5%和 96±1.5%的凋亡细胞。用 0.25 和 0.5 mg/mL 的 HTE 处理 HCT-116 细胞 3 h 分别导致 caspase-3 特异性底物的 38.9±1.5%和 57.2±3%的裂解。RT-PCR 分析显示 HTE 提取物对 Apaf-1 和 NOXA 的 mRNA 水平没有影响。此外，添加 0.125 mg/mL 和 0.25 mg/mL 的 HTE 24 h 明显减弱了 HCT-116 细胞的细胞周期进展机制。提取物的 GC/MS 分析鉴定出 21 种植物化学物质，它们是已知的凋亡诱导剂和细胞周期阻滞剂。所有检测到的化合物在 中都是新的。这些结果表明，HTE 诱导的人结肠细胞凋亡主要通过 caspase 依赖性途径介导。因此，HTE 似乎是治疗结肠癌的有效治疗剂。
