Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
Department of Life Sciences and Chemistry, Jacobs University Bremen, 28759 Bremen, Germany.
Food Res Int. 2019 Dec;126:108544. doi: 10.1016/j.foodres.2019.108544. Epub 2019 Sep 2.
Coffea arabica and Coffea canephora (Robusta coffee) are the most commonly consumed coffee varieties globally. In this contribution, NMR was used to confirm the coffee authenticity and LC-ESI-MS technique was employed to profile and quantify the most abundant chlorogenic acid in 54 different samples of the two coffee varieties from diverse origins. Significant variations were observed for feruloyl quinic acids, dicaffeoyl quinic acids and 5-sinapoylquinic acid while the mono-caffeoyl quinic acids showed no variation when the two coffee varieties were compared. Additionally isomer ratios were explored as a potential marker for coffee authenticity along with a thorough statistical evaluation of rather extensive data set. Robusta 5-CQA when compared with 3,4-DiCQA Robusta shows high positive correlation, similar high correlation coefficient was observed in 5-pCoQA Robusta when compared with 3-pCoQA as against the Arabica.
阿拉比卡咖啡（Arabica coffee）和罗布斯塔咖啡（Coffea canephora，又名“粗壮咖啡”）是全球最常消费的两种咖啡品种。在本研究中，我们采用核磁共振（NMR）来确认咖啡的真实性，并使用 LC-ESI-MS 技术对来自不同产地的 54 种不同阿拉比卡咖啡和罗布斯塔咖啡样品中的主要绿原酸进行分析和定量。在比较两种咖啡品种时，我们观察到阿魏酰奎宁酸、二咖啡酰奎宁酸和 5-芥子酰奎宁酸的含量存在显著差异，而单咖啡酰奎宁酸则没有差异。此外，我们还探索了异构体比例作为咖啡真实性的潜在标志物，并对相当广泛的数据集进行了全面的统计评估。与 3,4-二咖啡酰奎宁酸（3,4-DiCQA）相比，罗布斯塔 5-CQA 呈现出高度正相关，而与阿拉比卡相比，罗布斯塔 5-pCoQA 与 3-pCoQA 也呈现出相似的高相关系数。
