Departamento de Ingeniería Mecánica, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, Las Palmas, Spain.
Departamento de Ingeniería de Procesos, Universidad de Las Palmas de Gran Canaria, Campus Universitario de Tafira s/n, 35017, Las Palmas, Spain.
J Tissue Eng Regen Med. 2020 Feb;14(2):272-283. doi: 10.1002/term.2990. Epub 2019 Nov 26.
In this study, polylactic acid (PLA)-based composite scaffolds with calcium carbonate (CaCO ) and beta-tricalcium phosphate (β-TCP) were obtained by 3D printing. These structures were evaluated as potential 3D structures for bone tissue regeneration. Morphological, mechanical, and biological tests were carried out in order to compare the effect of each additive (added in a concentration of 5% w/w) and the combination of both (2.5% w/w of each one), on the PLA matrix. The scaffolds manufactured had a mean pore size between 400-425 μm and a porosity value in the range of 50-60%. According to the results, both additives promoted an increase of the porosity, hydrophilicity, and surface roughness of the scaffolds, leading to a significant improvement of the metabolic activity of human osteoblastic osteosarcoma cells. The best results in terms of cell attachment after 7 days were obtained for the samples containing CaCO and β-TCP particles due to the synergistic effect of both additives, which results in an increase in osteoconductivity and in a microporosity that favours cell adhesion. These scaffolds (PLA:CaCO :β-TCP 95:2.5:2.5) have suitable properties to be further evaluated for bone tissue engineering applications.
在这项研究中，通过 3D 打印获得了碳酸钙 (CaCO ) 和 β-磷酸三钙 (β-TCP) 增强的聚乳酸 (PLA) 基复合支架。这些结构被评估为潜在的骨组织再生 3D 结构。进行了形态学、力学和生物学测试，以比较每种添加剂（以 5%w/w 的浓度添加）及其组合（各添加 2.5%w/w）对 PLA 基质的影响。制造的支架的平均孔径在 400-425μm 之间，孔隙率在 50-60%范围内。根据结果，两种添加剂都促进了支架的孔隙率、亲水性和表面粗糙度的增加，从而显著提高了人成骨肉瘤细胞的代谢活性。在 7 天后，由于两种添加剂的协同作用，含有 CaCO 和β-TCP 颗粒的样品获得了最佳的细胞附着效果，因为这种协同作用增加了骨传导性和有利于细胞附着的微孔率。这些支架（PLA:CaCO :β-TCP 95:2.5:2.5）具有适合进一步评估骨组织工程应用的特性。
