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通过材料挤出3D打印评估添加剂对用于支架应用的新型基于PHB/PLA配方的细胞代谢活性的影响。

Evaluation of Additives on the Cell Metabolic Activity of New PHB/PLA-Based Formulations by Means of Material Extrusion 3D Printing for Scaffold Applications.

作者信息

Dominguez-Candela Ivan, Sempere-José Lluc, Sandoval-Perez Ignacio, Martínez-García Asunción

机构信息

AIJU Technological Institute for Children's Products & Leisure, 03440 Ibi, Spain.

出版信息

Polymers (Basel). 2024 Sep 30;16(19):2784. doi: 10.3390/polym16192784.

DOI:10.3390/polym16192784
PMID:39408494
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11478411/
Abstract

In this study, specific additives were incorporated in polyhydroxyalcanoate (PHB) and polylactic acid (PLA) blend to improve its compatibility, and so enhance the cell metabolic activity of scaffolds for tissue engineering. The formulations were manufactured through material extrusion (MEX) additive manufacturing (AM) technology. As additives, petroleum-based poly(ethylene) with glicidyl metacrylate (EGM) and methyl acrylate-co-glycidyl methacrylate (EMAG); poly(styrene-co-maleic anhydride) copolymer (Xibond); and bio-based epoxidized linseed oil (ELO) were used. On one hand, standard geometries manufactured were assessed to evaluate the compatibilizing effect. The additives improved the compatibility of PHB/PLA blend, highlighting the effect of EMAG and ELO in ductile properties. The processability was also enhanced for the decrease in melt temperature as well as the improvement of thermal stability. On the other hand, manufactured scaffolds were evaluated for the purpose of bone regeneration. The mean pore size and porosity exhibited values between 675 and 718 μm and 50 and 53%, respectively. According to the results, the compression stress was higher (11-13 MPa) than the required for trabecular bones (5-10 MPa). The best results in cell metabolic activity were obtained by incorporating ELO and Xibond due to the decrease in water contact angle, showing a stable cell attachment after 7 days of culture as observed in SEM.

摘要

在本研究中，将特定添加剂加入聚羟基脂肪酸酯（PHB）和聚乳酸（PLA）共混物中以改善其相容性，从而提高用于组织工程的支架的细胞代谢活性。这些配方是通过材料挤出（MEX）增材制造（AM）技术制造的。作为添加剂，使用了带有甲基丙烯酸缩水甘油酯（EGM）和丙烯酸甲酯-甲基丙烯酸缩水甘油酯共聚物（EMAG）的石油基聚乙烯；聚（苯乙烯-马来酸酐）共聚物（Xibond）；以及生物基环氧化亚麻籽油（ELO）。一方面，对制造的标准几何形状进行评估以评价增容效果。添加剂改善了PHB/PLA共混物的相容性，突出了EMAG和ELO对韧性的影响。由于熔体温度降低以及热稳定性提高，加工性能也得到了增强。另一方面，对制造的支架进行骨再生目的的评估。平均孔径和孔隙率分别在675至718μm和50至53%之间。根据结果，压缩应力（11 - 13MPa）高于松质骨所需的应力（5 - 10MPa）。由于水接触角减小，通过加入ELO和Xibond获得了细胞代谢活性方面的最佳结果，如扫描电子显微镜观察到的，培养7天后显示出稳定的细胞附着。

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