Imai N, Akimoto H, Oda M, Okazaki H, Ishibashi S, Kurokawa M
Department of Physiological Chemistry, Hiroshima University School of Medicine, Japan.
Mol Cell Biochem. 1988 May;81(1):37-41. doi: 10.1007/BF00225651.
Interactions between cations in modifying the binding of hexokinases I and II to mitochondria was examined with reference to the intracellular condition. Mitochondria-binding of either of hexokinases I and II, both prepared from mouse ascites ELD cells, was markedly increased by Mg2+ as has been known well. However, even in the absence of Mg2+, marked binding was attained by 100 mM K+ alone especially for hexokinase I, which seemed generally more ready to bind to mitochondria. On the other hand, the effect of Mg2+ to increase the binding was reduced by the addition of K+, and the decreasing effect of K+ was much more marked for hexokinase II than I. These results indicate that, in addition to Mg2+, monovalent cations as represented by K+, also have marked effect on the binding, and the effect is different for each hexokinases I and II, which may be responsible for the difference in the intracellular distribution between these hexokinases.
参照细胞内环境，研究了阳离子在调节己糖激酶I和II与线粒体结合中的相互作用。从小鼠腹水ELD细胞制备的己糖激酶I和II与线粒体的结合，如所知，均被Mg2+显著增强。然而，即使在没有Mg2+的情况下，仅100 mM K+就能实现显著结合，尤其是对于己糖激酶I，它似乎通常更容易与线粒体结合。另一方面，添加K+会降低Mg2+增加结合的效果，且K+对己糖激酶II的降低作用比对I更显著。这些结果表明，除了Mg2+之外，以K+为代表的单价阳离子对结合也有显著影响，且对己糖激酶I和II的影响不同，这可能是这些己糖激酶在细胞内分布差异的原因。
