Chen Huijie, Muhammad Ishfaq, Zhang Yue, Ren Yudong, Zhang Ruili, Huang Xiaodan, Diao Lei, Liu Haixin, Li Xunliang, Sun Xiaoqi, Abbas Ghulam, Li Guangxing
Key Laboratory for Laboratory Animals and Comparative Medicine of Heilongjiang Province, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
College of Biological and Pharmaceutical Engineering, Jilin Agricultural Science and Technology University, Jilin, China.
Front Pharmacol. 2019 Oct 29;10:1272. doi: 10.3389/fphar.2019.01272. eCollection 2019.
L., also known as , has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of on infectious bronchitis virus (IBV) were evaluated and for the first time. The results of experiments confirmed that the antiviral component of was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative messenger ribonucleic acid (mRNA) expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. HPE treatment at doses of 480-120 mg/kg for 5 days, reduced IBV induced injury in the trachea and kidney, moreover, reduced the mRNA expression level of IBV in the trachea and kidney . The mRNA expression levels of IL-6, tumor necrosis factor alpha (TNF-α), and nuclear factor kappa beta (NF-κB) significantly decreased, but melanoma differentiation-associated protein 5 (MDA5), mitochondrial antiviral signaling gene, interferon alpha (IFN-α), and interferon beta (IFN-β) mRNA levels significantly increased and . Our findings demonstrated that HPE had significant anti-IBV effects and , respectively. In addition, it is possible owing to up-regulate mRNA expression of type I interferon through the MDA5 signaling pathway and down-regulate mRNA expression of IL-6 and TNF-α the NF-κB signaling pathway. Moreover, the mainly active compositions of HPE analyzed by high-performance liquid chromatography/electrospray ionization-mass spectroscopy (ESI-MS) are hyperoside, quercitrin, quercetin, pseudohypericin, and hypericin, and a combination of these compounds could mediate the antiviral activities. This might accelerate our understanding of the antiviral effect of and provide new insights into the development of effective therapeutic strategies.
L.，也被称为 ，其化学成分和药理活性已得到充分研究。在本研究中，首次评估了 对传染性支气管炎病毒（IBV）的抗病毒活性。实验结果证实， 的抗病毒成分是乙酸乙酯萃取部分（HPE），结果表明，用HPE处理可显著降低IBV的相对信使核糖核酸（mRNA）表达和病毒滴度，并降低鸡胚肾（CEK）细胞中IBV的绿色阳性免疫荧光信号。以480 - 120 mg/kg的剂量给予HPE处理5天，可减轻IBV诱导的气管和肾脏损伤，此外，还可降低气管和肾脏中IBV的mRNA表达水平。白细胞介素-6（IL-6）、肿瘤坏死因子α（TNF-α）和核因子κB（NF-κB）的mRNA表达水平显著降低，但黑色素瘤分化相关蛋白5（MDA5）、线粒体抗病毒信号基因、干扰素α（IFN-α）和干扰素β（IFN-β）的mRNA水平显著升高 。我们的研究结果表明，HPE分别具有显著的抗IBV作用 。此外，可能是由于通过MDA5信号通路上调I型干扰素的mRNA表达，并通过NF-κB信号通路下调IL-6和TNF-α的mRNA表达。此外，通过高效液相色谱/电喷雾电离质谱（ESI-MS）分析，HPE的主要活性成分是金丝桃苷、槲皮苷、槲皮素、假金丝桃素和金丝桃素，这些化合物的组合可介导抗病毒活性。这可能会加速我们对 的抗病毒作用的理解，并为开发有效的治疗策略提供新的见解。
