Xinjiang Laboratory of Meat-and Milk-Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
PLoS One. 2019 Nov 18;14(11):e0223503. doi: 10.1371/journal.pone.0223503. eCollection 2019.
The aim of this research was to isolate bacteria capable of biotransforming daidzein from fresh feces from pregnant horses. A Hungate anaerobic roller tube was used for anaerobic culture. Single colonies were picked at random and incubated with daidzein. High performance liquid chromatography was used to detect whether the isolated bacteria were able to biotransform the substrate. A strain capable of reducing daidzein was selected and characterized using sequence analysis of 16S rDNA, and a phylogenetic tree was constructed. The morphological physiological and biochemical characteristics of the strain were investigated. A facultative anaerobic, Gram-positive bacterium capable of converting daidzein to dihydrodaidzein was isolated and named HXBM408 (MF992210). A BLAST search of HXBM408's 16S rDNA sequence against the GenBank database suggested that the strain has 99% similarity with Pediococcus acidilactici strain DSM (NR042057). The morphological, physiological, and biochemical characteristics of HXBM408 are very similar to those of Pediococcus. Based on these characteristics, the strain was identified as Pediococcus acidilactici. The bacterial strain HXBM408 isolated from the feces of pregnant horses was able to reduce the isoflavone daidzein to dihydrodaidzein.
本研究旨在从怀孕马的新鲜粪便中分离出能够转化大豆苷元的细菌。使用亨盖特厌氧滚管进行厌氧培养。随机挑取单菌落，并用大豆苷元孵育。采用高效液相色谱法检测分离出的细菌是否能够转化底物。选择能够还原大豆苷元的菌株，并通过 16S rDNA 序列分析进行表征，构建系统发育树。研究了菌株的形态、生理和生化特性。从怀孕马的粪便中分离到一株能够将大豆苷元转化为二氢大豆苷元的兼性厌氧、革兰氏阳性菌，命名为 HXBM408（MF992210）。对 HXBM408 的 16S rDNA 序列与 GenBank 数据库进行 BLAST 搜索表明，该菌株与乳球菌 DSM 株（NR042057）具有 99%的相似性。HXBM408 的形态、生理和生化特性与乳球菌非常相似。基于这些特征，该菌株被鉴定为嗜酸乳球菌。从怀孕马粪便中分离到的细菌菌株 HXBM408 能够将异黄酮大豆苷元还原为二氢大豆苷元。
