Weigand Michael R, Peng Yanhui, Batra Dhwani, Burroughs Mark, Davis Jamie K, Knipe Kristen, Loparev Vladimir N, Johnson Taccara, Juieng Phalasy, Rowe Lori A, Sheth Mili, Tang Kevin, Unoarumhi Yvette, Williams Margaret M, Tondella M Lucia
Division of Bacterial Disease, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Division of Bacterial Disease, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
mSystems. 2019 Nov 19;4(6):e00702-19. doi: 10.1128/mSystems.00702-19.
Whooping cough (pertussis), primarily caused by , has resurged in the United States, and circulating strains exhibit considerable chromosome structural fluidity in the form of rearrangement and deletion. The genus includes additional pathogenic species infecting various animals, some even causing pertussis-like respiratory disease in humans; however, investigation of their genome evolution has been limited. We studied chromosome structure in complete genome sequences from 167 species isolates, as well as 469 isolates, to gain a generalized understanding of rearrangement patterns among these related pathogens. Observed changes in gene order primarily resulted from large inversions and were only detected in species with genomes harboring multicopy insertion sequence (IS) elements, most notably and While genomes of contain >240 copies of IS, IS elements appear less numerous in other species and yield less chromosome structural diversity through rearrangement. These data were further used to predict all possible rearrangements between IS element copies present in genomes, revealing that only a subset is observed among circulating strains. Therefore, while it appears that rearrangement occurs less frequently in other species than in , these clinically relevant respiratory pathogens likely experience similar mutation of gene order. The resulting chromosome structural fluidity presents both challenges and opportunity for the study of respiratory pathogens. is the primary agent of whooping cough (pertussis). The genus includes additional pathogens of animals and humans, including some that cause pertussis-like respiratory illness. The chromosome of has previously been shown to exhibit considerable structural rearrangement, but insufficient data have prevented comparable investigation in related species. In this study, we analyze chromosome structure variation in several species to gain a generalized understanding of rearrangement patterns in this genus. Just as in , we observed inversions in other species that likely result from common mutational processes. We used these data to further predict additional, unobserved inversions, suggesting that specific genome structures may be preferred in each species.
百日咳主要由[具体病原体名称未给出]引起，在美国已再度流行，且流行菌株呈现出以重排和缺失形式存在的相当大的染色体结构流动性。[病原体所属属名未给出]属还包括感染各种动物的其他致病物种，有些甚至在人类中引起类似百日咳的呼吸道疾病；然而，对它们基因组进化的研究一直有限。我们研究了167株[病原体名称未给出]物种分离株以及469株[另一病原体名称未给出]分离株的全基因组序列中的染色体结构，以全面了解这些相关病原体之间的重排模式。观察到的基因顺序变化主要由大型倒位引起，并且仅在具有包含多拷贝插入序列（IS）元件的基因组的物种中检测到，最显著的是[具体物种名称未给出]和[另一具体物种名称未给出]。虽然[某物种名称未给出]的基因组含有>240个IS拷贝，但IS元件在其他物种中似乎数量较少，并且通过重排产生的染色体结构多样性也较少。这些数据进一步用于预测[某物种名称未给出]基因组中存在的IS元件拷贝之间的所有可能重排，结果表明在流行菌株中仅观察到一个子集。因此，虽然在其他物种中重排似乎比在[某物种名称未给出]中发生得更不频繁，但这些临床相关的呼吸道病原体可能经历类似的基因顺序突变。由此产生的染色体结构流动性给[病原体名称未给出]呼吸道病原体的研究带来了挑战和机遇。[病原体名称未给出]是百日咳的主要病原体。[病原体所属属名未给出]属包括感染动物和人类的其他病原体，包括一些引起类似百日咳的呼吸道疾病的病原体。先前已表明[某病原体名称未给出]的染色体表现出相当大的结构重排，但数据不足阻碍了对相关物种进行类似的研究。在本研究中，我们分析了几种[病原体名称未给出]物种的染色体结构变异，以全面了解该属中的重排模式。正如在[某病原体名称未给出]中一样，我们在其他物种中观察到可能由常见突变过程导致的倒位。我们利用这些数据进一步预测其他未观察到的倒位，表明每个物种可能偏好特定的基因组结构。
