Gu Ran
Department of Biochemistry and Molecular Medicine, School of Medicine, University of California at Davis, Sacramento, CA, USA.
Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children-Northern California, UC Davis School of Medicine, Sacramento, CA, USA.
Methods Mol Biol. 2020;2080:135-145. doi: 10.1007/978-1-4939-9936-1_12.
Osteoclasts are the only cells that are capable of resorbing bones, and they are involved in multiple diseases and disorders. This chapter will describe several in vitro osteoclastogenesis methods, which allows further investigation of molecular mechanisms of osteoclastogenesis in normal physiological and disease conditions. This chapter includes a protocol for isolating osteoclast progenitors from mouse bone marrow and human peripheral blood, as well as obtaining murine osteoblasts for the coculture system. Furthermore, culture and identification of multinucleated osteoclasts in vitro is also described in this chapter.
破骨细胞是唯一能够吸收骨骼的细胞，它们参与多种疾病和病症。本章将描述几种体外破骨细胞生成方法，这些方法有助于在正常生理和疾病条件下进一步研究破骨细胞生成的分子机制。本章包括从小鼠骨髓和人外周血中分离破骨细胞祖细胞的方案，以及为共培养系统获取小鼠成骨细胞的方案。此外，本章还描述了体外多核破骨细胞的培养和鉴定。
