人血制品中前激肽释放酶激活剂的测定
The assay of prekallikrein activator in human blood products.
作者信息
Snape T J, Griffith D, Vallet L, Wesley E D
出版信息
Dev Biol Stand. 1979;44:115-20.
An assay for the determination of prekallikrein activator (PKA) in human blood products is described. Kallikrein released from a prekallikrein substrate preparation by PKA in the sample is determined using the synthetic substrate S-2302 (H-D-Pro-Phe-Arg-pNA). The PKA content of the sample is quantitated by interpolation from a standard line obtained using a reference preparation of plasma protein fraction (PPF). Endogenous kallikrein is estimated and an adjustment made for its contribution to the apparent PKA content of the sample. The results of determinations on a number of PPF, immunoglobulin and coagulation factor preparations are presented and discussed.
本文描述了一种用于测定人血制品中前激肽释放酶激活剂（PKA）的检测方法。使用合成底物S-2302（H-D-脯氨酸-苯丙氨酸-精氨酸-对硝基苯胺）来测定样品中PKA从激肽释放酶原底物制剂释放出的激肽释放酶。通过使用血浆蛋白组分（PPF）参比制剂获得的标准曲线进行内插法来定量样品中的PKA含量。估算内源性激肽释放酶，并对其对样品表观PKA含量的贡献进行校正。给出并讨论了对多种PPF、免疫球蛋白和凝血因子制剂的检测结果。
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