利用近红外荧光探针实现内源性半胱氨酸、同型半胱氨酸和谷胱甘肽的光驱动可视化。
Light-driven visualization of endogenous cysteine, homocysteine, and glutathione using a near-infrared fluorescent probe.
机构信息
Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.
出版信息
J Mater Chem B. 2019 Dec 11;7(48):7723-7728. doi: 10.1039/c9tb01645g.
Herein, we presented a hydrosoluble triple-site and triple-excitation alternative NIR fluorescent probe for visualization of endogenous biothiols in phosphate-buffered saline (pH 7.4, 10 mM). Upon irradiation using different excitation light, probe 1 exhibited different fluorescence responses upon the addition of Cys, Hcy, and GSH: λex = 419 nm, λem = 498 nm; λex = 518 nm, λem = 573, 616, 727, and 783 nm; λex = 555 nm, λem = 612 and 727 nm, respectively. Furthermore, 1 was favourably applied for bioimaging endogenous Cys, Hcy, and GSH in A375 cells through well-defined blue-green-red emission channels.
在这里，我们提出了一种水溶性三靶点和三激发近红外荧光探针，用于在磷酸盐缓冲盐水（pH 7.4，10 mM）中可视化内源性生物硫醇。当使用不同的激发光照射时，探针 1 在加入半胱氨酸、同型半胱氨酸和谷胱甘肽后表现出不同的荧光响应：λex = 419nm，λem = 498nm；λex = 518nm，λem = 573、616、727 和 783nm；λex = 555nm，λem = 612 和 727nm。此外，1 还通过明确定义的蓝-绿-红发射通道，有利地应用于 A375 细胞中内源性 Cys、Hcy 和 GSH 的生物成像。
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