da Fonseca Iwahara Lucas Kiyoshi, de Paoli Flavia, da Fonseca Adenilson de Souza
Instituto de Biociências, Universidade Federal do Estado do Rio de Janeiro, Avenida Pasteur, 296, Urca, Rio de Janeiro, 22290240, Brazil.
Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n - Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais, 36036900, Brazil.
J Lasers Med Sci. 2019 Summer;10(3):157-162. doi: 10.15171/jlms.2019.25. Epub 2019 Jul 6.
Low-level lasers are successfully used to prevent and treat diseases in soft oral and bone tissues, particularly diseases in oral cavity caused by chemotherapy and radiotherapy in oncology. However, controversy exists as to whether these lasers induce molecular side effects, mainly on DNA. The aim of this work was to assess the effects of low-power lasers on mutant cells in DNA repair. wild type cultures as well as those lacking recombination DNA repair ( ) and la SOS responses ( ) irradiated with lasers at different energy densities, powers, and emission modes for cell viability and morphology assessment were used in this study. Laser irradiation: () did not affect cell viability of non-mutant and cells but decreased viability in cultures; () altered morphology of wild type and , depending on the energy density, power, emission mode, and wavelength. Results show that low-level lasers have lethal effects on both recombination DNA repair and SOS response bacterial cells but do not induce morphological modifications in these cells.
低强度激光已成功用于预防和治疗口腔软组织及骨组织疾病，尤其是肿瘤学中化疗和放疗引起的口腔疾病。然而，关于这些激光是否会引发分子副作用，主要是对DNA的影响，仍存在争议。这项工作的目的是评估低功率激光对DNA修复中的突变细胞的影响。本研究使用了野生型培养物以及缺乏重组DNA修复（ ）和SOS反应（ ）的培养物，用不同能量密度、功率和发射模式的激光照射，以评估细胞活力和形态。激光照射：（ ）不影响非突变和 细胞的细胞活力，但会降低 培养物中的活力；（ ）根据能量密度、功率、发射模式和波长改变野生型和 的形态。结果表明，低强度激光对重组DNA修复和SOS反应细菌细胞均有致死作用，但不会在这些细胞中诱导形态学改变。
