Centre for Aquatic Pollution Identification and Management, The University of Melbourne, Parkville, Victoria, Australia.
Aquatic Environmental Stress Research Group, School of Science, Royal Melbourne Institute of Technology University, Melbourne, Victoria, Australia.
Environ Toxicol Chem. 2020 Mar;39(3):595-603. doi: 10.1002/etc.4640. Epub 2020 Jan 24.
Per- and poly-fluoroalkyl substances (PFAS) are ubiquitously distributed throughout aquatic environments and can bioaccumulate in organisms. We examined dietary uptake and depuration of a mixture of 3 PFAS: perfluorooctanoic acid (PFOA; C HF O ), perfluorooctane sulfonate (PFOS; C HF SO ), and hexafluoropropylene oxide dimer acid (HPFO-DA; C HF O ; trade name GenX). Benthic fish (blue spot gobies, Pseudogobius sp.) were fed contaminated food (nominal dose 500 ng g ) daily for a 21-d uptake period, followed by a 42-d depuration period. The compounds PFOA, linear-PFOS (linear PFOS), and total PFOS (sum of linear and branched PFOS) were detected in freeze-dried fish, whereas GenX was not, indicating either a lack of uptake or rapid elimination (<24 h). Depuration rates (d ) were 0.150 (PFOA), 0.045 (linear-PFOS), and 0.042 (linear+branched-PFOS) with corresponding biological half-lives of 5.9, 15, and 16 d, respectively. The PFOS isomers were eliminated differently, resulting in enrichment of linear-PFOS (70-90%) throughout the depuration period. The present study is the first reported study of GenX dietary bioaccumulation potential in fish, and the first dietary study to investigate uptake and depuration of multiple PFASs simultaneously, allowing us to determine that whereas PFOA and PFOS accumulated as expected, GenX, administered in the same way, did not appear to bioaccumulate. Environ Toxicol Chem 2020;39:595-603. © 2019 SETAC.
全氟和多氟烷基物质（PFAS）广泛分布于水生环境中，并能在生物体内生物累积。我们研究了 3 种 PFAS（全氟辛酸（PFOA；C 8 HF O ）、全氟辛烷磺酸（PFOS；C 8 HF SO ）和六氟丙烯氧化物二聚酸（HPFO-DA；C 8 HF O ；商品名 GenX））混合物的经口摄入和消除情况。底栖鱼类（蓝斑拟鱚，Pseudogobius sp.）每天喂食受污染的食物（名义剂量 500ng/g），持续 21 天的摄入期，然后是 42 天的消除期。在冻干鱼中检测到化合物 PFOA、线性-PFOS（线性 PFOS）和总 PFOS（线性和支链 PFOS 的总和），但未检测到 GenX，这表明要么是摄入不足，要么是消除迅速（<24 小时）。消除率（d）分别为 0.150（PFOA）、0.045（线性-PFOS）和 0.042（线性+支链-PFOS），相应的生物半衰期分别为 5.9、15 和 16 天。PFOS 异构体的消除方式不同，导致线性-PFOS 在整个消除期内富集（70-90%）。本研究是首次报道 GenX 在鱼类经口生物累积潜力的研究，也是首次同时研究多种 PFAS 经口摄入和消除的研究，使我们能够确定 PFOA 和 PFOS 如预期般累积，而以相同方式给予的 GenX 似乎并未生物累积。Environ Toxicol Chem 2020；39：595-603。©2019 SETAC。
