J Am Assoc Lab Anim Sci. 2020 Jan 1;59(1):67-73. doi: 10.30802/AALAS-JAALAS-19-000053. Epub 2019 Nov 21.
Appropriate aseptic technique is a crucial component of rodent survival surgery. Ease of technique, surgical space constraint, batch surgery, and cost are factors that may affect researcher compliance with appropriate aseptic technique. The first part of this study compared 3 antiseptic preparation agents with the standard triplicate application of povidone-iodine and alcohol. Euthanized mice ( = 40) were shaved on the dorsum, and culture swabs were taken for RODAC plating and bacterial identification. Shaved sites were prepared by using one of the 4 antiseptic preparation agents. Culture samples were obtained immediately and at 20 min after antiseptic preparation. In the 2nd part of the study, 8 mice ( = 2 per group) were prepared for a survival surgical procedure by using one of the 4 antiseptic preparation agents to evaluate whether the antiseptic preparation agents caused skin irritation or impaired healing. Results from this study indicated that all 3 of the antiseptic agents evaluated were equally effective at reducing bacterial populations immediately and at 20 min after preparation. Histopathologic examination of the incision sites revealed signs of normal healing without lesions adjacent to the incision site. We conclude that all 3 of the products evaluated are comparable to traditional povidone-iodine and alcohol as agents for aseptic preparation of surgical sites.
适当的无菌技术是啮齿动物存活手术的关键组成部分。技术的简便性、手术空间的限制、批量手术和成本是可能影响研究人员遵守适当无菌技术的因素。本研究的第一部分比较了 3 种消毒准备剂与重复应用聚维酮碘和酒精的标准方法。对安乐死的小鼠（=40）进行背部剃毛，并用 RODAC 平板进行培养拭子取样以进行细菌鉴定。使用 4 种消毒准备剂中的一种对剃毛部位进行准备。立即以及在消毒准备 20 分钟后采集培养样本。在研究的第二部分中，通过使用 4 种消毒准备剂中的一种对 8 只小鼠（=每组 2 只）进行存活手术准备，以评估消毒准备剂是否会引起皮肤刺激或影响愈合。本研究的结果表明，在准备后立即和 20 分钟时，所有 3 种评估的消毒剂在减少细菌数量方面同样有效。切口部位的组织病理学检查显示出正常愈合的迹象，切口部位无病变。我们得出结论，评估的所有 3 种产品与传统的聚维酮碘和酒精一样，可作为手术部位无菌准备的试剂。
