Bozzetto Lutgarda, Berntzen Bram J, Kaprio Jaakko, Rissanen Aila, Taskinen Marja-Riitta, Pietiläinen Kirsi H
Department of Clinical Medicine and Surgery, Federico II University Naples, Italy.
Obesity Research Unit, Research Program for Clinical and Molecular Metabolism, Faculty of Medicine, University of Helsinki, Helsinki, Finland.
Nutr Metab Cardiovasc Dis. 2020 Mar 9;30(3):459-466. doi: 10.1016/j.numecd.2019.10.005. Epub 2019 Oct 15.
Plasma apolipoprotein C3 (ApoC3) is associated with higher plasma triglyceride and type 2 diabetes incidence. We evaluated whether body mass index (BMI) or glucose metabolism were associated with ApoC3 in healthy monozygotic (MZ) twins.
Forty-seven MZ twin-pairs (20 man, 27 women), aged 23-42 years, were divided in subgroups according to discordance or concordance for (a) BMI (within-pair difference (Δ) in BMI≥3.0 or<3.0 kg/m), or (b) 2-h glucose iAUC, during oral glucose tolerance test (ΔGlucose iAUC ≥97.5 or<97.5 mmol × 120 minutes). Within these discordant or concordant subgroups, we tested (Wilcoxon signed-rank test) co-twin differences in ApoC3, adiposity measures, insulin-resistance and beta-cell function indices, and plasma and lipoprotein lipids. In BMI-Discordant (p = 0.92) or BMI-Concordant (p = 0.99) subgroups, ApoC3 did not differ between leaner and heavier co-twins. In the Glucose-Discordant subgroup, ApoC3 was significantly higher in twins with higher Glucose iAUC than in their co-twins with the lower Glucose iAUC (10.03 ± 0.78 vs. 8.48 ± 0.52 mg/dl; M ± SE; p = 0.032). Co-twins with higher Glucose iAUC also had higher waist circumference, body fat percentage, liver fat content, worse insulin-sensitivity and beta-cell function and higher cholesterol and triglyceride in plasma VLDL, IDL, and LDL. In Glucose-Concordant twin-pairs, no significant differences were observed in the explored variables. In all twin-pairs, ΔApoC3 correlated with Δ in lipids and glucose metabolism variables, the closest relationship being between ΔApoC3 and ΔVLDL triglyceride (r = 0.74, p < 0.0001).
While ApoC3 was not related to acquired differences in BMI, it associated with early dysregulation of glucose metabolism independently of obesity and genetic background.
血浆载脂蛋白C3（ApoC3）与较高的血浆甘油三酯水平及2型糖尿病发病率相关。我们评估了在健康的同卵双胞胎中，体重指数（BMI）或糖代谢是否与ApoC3有关。
47对年龄在23至42岁之间的同卵双胞胎（20对男性，27对女性），根据（a）BMI（双胞胎间BMI差值（Δ）≥3.0或<3.0 kg/m²）或（b）口服葡萄糖耐量试验期间的2小时葡萄糖iAUC（Δ葡萄糖iAUC≥97.5或<97.5 mmol×120分钟）的不一致或一致情况分为亚组。在这些不一致或一致的亚组中，我们测试了（Wilcoxon符号秩检验）双胞胎在ApoC3、肥胖指标、胰岛素抵抗和β细胞功能指标以及血浆和脂蛋白脂质方面的差异。在BMI不一致（p = 0.92）或BMI一致（p = 0.99）的亚组中，较瘦和较胖的双胞胎之间ApoC3没有差异。在葡萄糖不一致亚组中，葡萄糖iAUC较高的双胞胎的ApoC3显著高于其葡萄糖iAUC较低的双胞胎（10.03±0.78 vs. 8.48±0.52 mg/dl；均值±标准误；p = 0.032）。葡萄糖iAUC较高的双胞胎还具有更高的腰围、体脂百分比、肝脏脂肪含量、更差的胰岛素敏感性和β细胞功能，以及血浆极低密度脂蛋白（VLDL）、中间密度脂蛋白（IDL）和低密度脂蛋白（LDL）中更高的胆固醇和甘油三酯。在葡萄糖一致的双胞胎对中，在所探索的变量中未观察到显著差异。在所有双胞胎对中，ΔApoC3与脂质和糖代谢变量的Δ相关，最密切的关系是ΔApoC3与ΔVLDL甘油三酯之间的关系（r = 0.74，p < 0.0001）。
虽然ApoC3与BMI的后天差异无关，但它与葡萄糖代谢的早期失调相关，且独立于肥胖和遗传背景。
