Horng Yi-Bing, Yu Yu-Hsiang, Dybus Andrzej, Hsiao Felix Shih-Hsiang, Cheng Yeong-Hsiang
Department of Biotechnology and Animal Science, National Ilan University, Yilan City, 26047, Yilan County, Taiwan.
Laboratory of Molecular Cytogenetics, Department of Genetics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland.
AMB Express. 2019 Nov 21;9(1):188. doi: 10.1186/s13568-019-0914-2.
Swine dysentery and necrotic enteritis are a bane to animal husbandry worldwide. Some countries have already banned the use of antibiotics as growth promoters in animal production. Surfactin is a potential alternative to antibiotics and antibacterial agents. However, the antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens are still poorly understood. In the current study, the antibacterial effects of surfactin produced from Bacillus subtilis and Bacillus licheniformis on B. hyodysenteriae and C. perfringens were evaluated. Results showed that multiple surfactin isoforms were detected in B. subtilis, while only one surfactin isoform was detected in B. licheniformis fermented products. The surfactin produced from B. subtilis exhibited significant antibacterial activity against B. hyodysenteriae compared with surfactin produced from B. licheniformis. B. subtilis-derived surfactin could inhibit bacterial growth and disrupt the morphology of B. hyodysenteriae. Furthermore, the surfactin produced from B. subtilis have the highest activity against C. perfringens growth. In contrast, B. licheniformis fermented product-derived surfactin had a strong bacterial killing activity against C. perfringens compared with surfactin produced from B. subtilis. These results together suggest that Bacillus species-derived surfactin have potential for development as feed additives and use as a possible substitute for antibiotics to prevent B. hyodysenteriae and C. perfringens-associated disease in the animal industry.
猪痢疾和坏死性肠炎是全球畜牧业的一大祸害。一些国家已经禁止在动物生产中使用抗生素作为生长促进剂。表面活性素是抗生素和抗菌剂的一种潜在替代品。然而，芽孢杆菌属来源的表面活性素对猪痢疾短螺旋体和产气荚膜梭菌的抗菌活性仍知之甚少。在本研究中，评估了枯草芽孢杆菌和地衣芽孢杆菌产生的表面活性素对猪痢疾短螺旋体和产气荚膜梭菌的抗菌作用。结果表明，在枯草芽孢杆菌中检测到多种表面活性素异构体，而在地衣芽孢杆菌发酵产物中仅检测到一种表面活性素异构体。与地衣芽孢杆菌产生的表面活性素相比，枯草芽孢杆菌产生的表面活性素对猪痢疾短螺旋体表现出显著的抗菌活性。枯草芽孢杆菌来源的表面活性素可抑制细菌生长并破坏猪痢疾短螺旋体的形态。此外，枯草芽孢杆菌产生的表面活性素对产气荚膜梭菌的生长具有最高活性。相比之下，与枯草芽孢杆菌产生的表面活性素相比，地衣芽孢杆菌发酵产物来源的表面活性素对产气荚膜梭菌具有较强的杀菌活性。这些结果共同表明，芽孢杆菌属来源的表面活性素具有开发为饲料添加剂的潜力，并可作为抗生素的可能替代品，用于预防动物行业中猪痢疾短螺旋体和产气荚膜梭菌相关疾病。
