HSPA8 在腰椎间盘髓核中的表达及其对退变程度的影响。

Expression of HSPA8 in Nucleus Pulposus of Lumbar Intervertebral Disc and Its Effect on Degree of Degeneration.

机构信息

Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University, 16 Jiangsu Street, Qingdao, Shandong, People's Republic of China.

Department of Minimally Invasive Spine Surgery, Qingdao Municipal Hospital, East Branch, Qingdao, Shandong, People's Republic of China.

出版信息

Adv Ther. 2020 Jan;37(1):390-401. doi: 10.1007/s12325-019-01136-9. Epub 2019 Nov 21.

DOI:10.1007/s12325-019-01136-9

PMID:31755037

Abstract

INTRODUCTION

This study aimed to investigate the expression of a 70-kDa heat shock protein [heat shock 70-kDa protein 8 (HSPA8)/heat shock protein 70 (Hsc70)] in human degenerative lumbar intervertebral discs and its relationship with the degree of degeneration of human intervertebral discs.

METHODS

A total of 72 cases of lumbar intervertebral disc nucleus pulposus tissues were collected. Among these, 18 cases of nucleus pulposus tissue were assigned to the control group, while 54 cases of nucleus pulposus tissues were assigned to the experimental group. According to the preoperative MRI, cases in the experimental group were further divided into three groups: protrusion group (n = 18), extrusion group (n = 18), and sequestration group (n = 18). Western blot was performed to determine the relative expression of HSPA8 in the nucleus pulposus in each group. Hematoxylin and eosin staining was performed to determine the number of nucleus pulposus cells, morphological differences, and cell densities of the degenerated intervertebral discs and normal intervertebral discs. Immunohistochemistry was performed to determine the expression of HSPA8 in nucleus pulposus tissues in each group.

RESULTS

Hematoxylin and eosin staining results: There were significant differences in cell morphology and number between the control group and the experimental group. Furthermore, there were significant differences in cell density (F = 936.80, P < 0.01). Immunohistochemistry results: HSPA8 was expressed in lumbar intervertebral disc nucleus pulposus tissues, and its expression of gradually decreased with the severity of the disease, and the differences were significant (F = 2110.43, P < 0.01). Western blot results: The expression of HSPA8 in human degenerative nucleus pulposus tissues gradually decreased, and the differences were significant (F = 1841.72, P < 0.01).

CONCLUSION

HSPA8 is stably expressed in human intervertebral disc nucleus pulposus tissues, and its expression is associated with the degree of intervertebral disc degeneration.

摘要

简介

本研究旨在探讨 70kDa 热休克蛋白（热休克 70kDa 蛋白 8 [HSPA8]/热休克蛋白 70 [Hsc70]）在人退变腰椎间盘组织中的表达及其与人类椎间盘退变程度的关系。

方法

共收集 72 例腰椎间盘髓核组织。其中，18 例髓核组织归入对照组，54 例髓核组织归入实验组。根据术前 MRI，实验组病例进一步分为突出组（n=18）、脱出组（n=18）和游离组（n=18）。采用 Western blot 法测定各组髓核中 HSPA8 的相对表达。苏木精-伊红染色法测定退变椎间盘与正常椎间盘的髓核细胞数、形态差异及细胞密度。免疫组化法测定各组髓核组织中 HSPA8 的表达。

结果

苏木精-伊红染色结果：对照组与实验组细胞形态和数量存在显著差异（F=936.80，P<0.01）。细胞密度差异有统计学意义（F=936.80，P<0.01）。免疫组化结果：HSPA8 在腰椎间盘髓核组织中表达，其表达随病情严重程度逐渐降低，差异有统计学意义（F=2110.43，P<0.01）。Western blot 结果：人退变髓核组织中 HSPA8 表达逐渐降低，差异有统计学意义（F=1841.72，P<0.01）。