Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China.
Int J Biol Macromol. 2020 Aug 1;156:1112-1125. doi: 10.1016/j.ijbiomac.2019.11.142. Epub 2019 Nov 19.
The antioxidant activities of polysaccharides from the fruiting body (PFB), extracellular polysaccharides (EPS) and intracellular polysaccharides (IPS) from Agaricus bitorquis (QuéL.) Sacc. Chaidam ZJU-CDMA-12 in vitro were compared. IPS showed stronger antioxidant activities than PFB and EPS in vitro. Further purification and structure analyses indicated that IPS mainly consisted of three fractions (IPS-I, IPS-II and IPS-III). FT-IR and NMR data indicated that IPS was mainly composed of (1 → 6)-linked α-d-glucose. There are significant differences of antioxidant activities among IPS-I, IPS-II and IPS-III fractions in vitro, and IPS-III showed stronger antioxidant activity than IPS-I and IPS-II. IPS-III also possesses a potent antioxidant ability inside HepG2 cells, and it could protect HepG2 cells from HO-induced cytotoxicity by scavenging overproduced cellular ROS and inhibiting SOD, CAT and GSH depletion to weaken lipid peroxidation. These findings suggested that IPS-III could be a novel antioxidant and that it could afford protection against HO-induced cytotoxicity and oxidative stress.
比较了白灵侧耳（Agaricus bitorquis（QuéL.）Sacc. Chaidam ZJU-CDMA-12）子实体多糖（PFB）、胞外多糖（EPS）和胞内多糖（IPS）的体外抗氧化活性。IPS 显示出比 PFB 和 EPS 更强的体外抗氧化活性。进一步的纯化和结构分析表明，IPS 主要由三个部分（IPS-I、IPS-II 和 IPS-III）组成。FT-IR 和 NMR 数据表明 IPS 主要由（1→6）-连接的α-d-葡萄糖组成。在体外，IPS-I、IPS-II 和 IPS-III 三个部分的抗氧化活性存在显著差异，IPS-III 显示出比 IPS-I 和 IPS-II 更强的抗氧化活性。IPS-III 在 HepG2 细胞内也具有很强的抗氧化能力，它可以通过清除过量产生的细胞 ROS 和抑制 SOD、CAT 和 GSH 的消耗来减弱脂质过氧化，从而保护 HepG2 细胞免受 HO 诱导的细胞毒性。这些发现表明 IPS-III 可能是一种新型的抗氧化剂，能够提供对 HO 诱导的细胞毒性和氧化应激的保护。
