Virology and Cell Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand.
Virology and Cell Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand.
Virology. 2020 Jan 15;540:45-56. doi: 10.1016/j.virol.2019.11.007. Epub 2019 Nov 7.
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine deltacoronavirus (PDCoV) share tropism for swine intestinal epithelial cells. Whether mixing of viral components during co-infection alters pathogenic outcomes or viral replication is not known. In this study, we investigated how different coronavirus nucleocapsid (CoV N) proteins interact and affect PEDV replication. We found that PDCoV N and TGEV N can competitively interact with PEDV N. However, the presence of PDCoV or TGEV N led to very different outcomes on PEDV replication. While PDCoV N significantly suppresses PEDV replication, overexpression of TGEV N, like that of PEDV N, increases production of PEDV RNA and virions. Despite partial interchangeability in nucleocapsid oligomerization and viral RNA synthesis, endogenous PEDV N cannot be replaced in the production of infectious PEDV particles. Results from this study give insights into functional compatibilities and evolutionary relationship between CoV viral proteins during viral co-infection and co-evolution.
猪流行性腹泻病毒（PEDV）、传染性胃肠炎病毒（TGEV）和猪德尔塔冠状病毒（PDCoV）都具有猪肠道上皮细胞的嗜性。在共感染期间，病毒成分的混合是否改变了致病结果或病毒复制尚不清楚。在这项研究中，我们研究了不同的冠状病毒核衣壳（CoV N）蛋白如何相互作用并影响 PEDV 的复制。我们发现 PDCoV N 和 TGEV N 可以与 PEDV N 竞争相互作用。然而，PDCoV 或 TGEV N 的存在对 PEDV 复制的结果却大不相同。虽然 PDCoV N 显著抑制 PEDV 复制，但 TGEV N 的过表达，就像 PEDV N 一样，增加了 PEDV RNA 和病毒粒子的产生。尽管在核衣壳寡聚化和病毒 RNA 合成方面具有部分可互换性，但内源性 PEDV N 不能替代感染性 PEDV 颗粒的产生。这项研究的结果深入了解了病毒共感染和共同进化过程中 CoV 病毒蛋白之间的功能兼容性和进化关系。
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