School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China; Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, People's Republic of China.
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, People's Republic of China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Dec 1;1133:121848. doi: 10.1016/j.jchromb.2019.121848. Epub 2019 Nov 1.
Inflammatory bowel disease (IBD) is often accompanied by metabolic imbalance and Berberine can relieve the symptoms of IBD, but the mechanism is still unclear. To explore the relationship between IBD, metabolism and Berberine, dextran sulfate sodium-induced ulcerative colitis (UC) model was built and urine and feces samples were analyzed with ultra-performance liquid chromatography combined with quadrupole-time-of-flight mass spectrometry, followed by multivariate statistical analyses. Targeted metabolomics was applied to verify and supplement the result of amino acids tested by non-targeted metabolomics. The study found that Berberine could ameliorate UC and improve metabolic disorders. The level of 4 metabolites increased and 35 decreased in urine and these metabolites mainly belong to amino acid, glucide, organic acid and purine. Besides, Berberine could reduce the level of 5 metabolites and raise the level of 7 metabolites in feces, which mainly belong to amino acid and lipid. Additionally, these altered metabolites were mainly related to amino acids metabolism, purine metabolism, vitamin metabolism, lipid metabolism and citrate cycle pathways. Furthermore, microbiome metabolism may be regulated by Berberine in UC. In general, this study provides a useful approach for exploring the mechanism of Berberine in the treatment of UC from the perspective of metabolomics.
炎症性肠病（IBD）常伴有代谢失衡，小檗碱可缓解 IBD 症状，但机制尚不清楚。为了探讨 IBD、代谢和小檗碱之间的关系，建立了葡聚糖硫酸钠诱导的溃疡性结肠炎（UC）模型，并用超高效液相色谱-四极杆飞行时间质谱联用进行尿液和粪便样本分析，然后进行多元统计分析。应用靶向代谢组学验证和补充非靶向代谢组学检测到的氨基酸结果。研究发现，小檗碱可改善 UC 并改善代谢紊乱。尿液中 4 种代谢物水平升高，35 种代谢物水平降低，这些代谢物主要属于氨基酸、糖、有机酸和嘌呤。此外，小檗碱可降低粪便中 5 种代谢物的水平，提高 7 种代谢物的水平，主要属于氨基酸和脂质。此外，这些改变的代谢物主要与氨基酸代谢、嘌呤代谢、维生素代谢、脂质代谢和柠檬酸循环途径有关。此外，小檗碱可能通过调节肠道微生物组代谢来调节 UC。总的来说，本研究从代谢组学角度为探讨小檗碱治疗 UC 的机制提供了一种有用的方法。
