Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, India.
Department of Food Science and Technology, Guru Nanak Dev University, Amritsar 143005, India.
Int J Biol Macromol. 2020 Jun 15;153:962-970. doi: 10.1016/j.ijbiomac.2019.10.225. Epub 2019 Nov 20.
The objective of this study was to evaluate the impact of sprouting (0, 12, 24, 36, 48 hr) on enzyme activity, protein solubility, dough mixing behavior, anti-nutritional components and in vitro starch and protein digestibility of minor millets. Sprouting decreased starch, fat, protein and ash contents while the dietary fiber content, amylase and protease activity significantly (p < 0.05) increased. Sprouting promoted a significant (p < 0.05) decrease in anti-nutritonal components like phytic acid and condensed tannin. The protein solubility enhanced that also resulted in improved protein digestibility (in vitro) of sprouted millets. Sprouting exerted a significant (p < 0.05) effect on mixolab dough rheology indicating a decrease in dough consistency (C1), peak and final viscosity whilst protein weakening and breakdown increased (p < 0.05). This process produced an increase in free glucose thereby improving rapidly digestible starch and increasing glycemic index. A significant (p < 0.05) reduction in resistant starch along with slowly digestible starch was promoted during sprouting. In conclusion, sprouting provides millet flours with modified nutritional quality and better technofunctional properties than the raw flours that may suggest their potential utilization in complementary food formulations and baked products.
本研究旨在评估发芽(0、12、24、36、48 小时)对微量元素酶活性、蛋白质溶解度、面团混合行为、抗营养成分以及体外淀粉和蛋白质消化率的影响。发芽使淀粉、脂肪、蛋白质和灰分含量降低,而膳食纤维含量、淀粉酶和蛋白酶活性显著(p<0.05)增加。发芽促进了抗营养成分如植酸和单宁的显著(p<0.05)减少。蛋白质溶解度提高,也导致发芽小米的体外蛋白质消化率提高。发芽对混合流变仪面团流变学产生了显著(p<0.05)的影响,表明面团稠度(C1)、峰值和最终黏度降低,而蛋白质弱化和分解增加(p<0.05)。这个过程产生了更多的游离葡萄糖,从而提高了快速消化淀粉的含量,增加了血糖指数。发芽过程中,抗性淀粉和缓慢消化淀粉显著(p<0.05)减少。总之,发芽使小米面粉具有改良的营养品质和更好的技术功能特性,优于原面粉,这可能表明它们在补充食品配方和烘焙产品中的潜在应用。