College of Life Sciences, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
College of Life Sciences, Liaoning Normal University, Dalian, 116081, China; Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
Fish Shellfish Immunol. 2020 Jan;96:336-349. doi: 10.1016/j.fsi.2019.11.037. Epub 2019 Nov 20.
Tumor necrosis factor receptor superfamilies (TNFRSF) are one of essential cytokines and can trigger inflammation, apoptosis, participating lymphocyte homeostasis and tissue development in vertebrates. To gain insights into the evolution and characterization of tnfr genes in lamprey, a jawless vertebrate, we performed a genome-wide and transcriptome survey and identified 7 tnfr genes in the lamprey (Lethenteron reissneri) database. Based on the molecular phylogenetic analysis, 7 L-tnfr genes are divided into three different clusters, and multiple members of tnfr genes family have appeared in lamprey. Meanwhile, protein domains and motifs analysis reveals that TNFRSF are conserved and have typical cysteine-rich domains (CRDs). Synteny results indicates that the L-tnfr neighborhood genes have taken place great changes compared to jawed vertebrates. Real-time quantitative results demonstrate that tnfr gene family plays an important role in the immune defense. This study has a new understanding for origin and evolution of the tnfr gene family in different vertebrates.
肿瘤坏死因子受体超家族（TNFRSF）是一类重要的细胞因子，能够在脊椎动物中引发炎症、细胞凋亡、参与淋巴细胞稳态和组织发育。为了深入了解无颚脊椎动物七鳃鳗中 tnfr 基因的进化和特征，我们进行了全基因组和转录组调查，在七鳃鳗（Lethenteron reissneri）数据库中鉴定出 7 个 tnfr 基因。基于分子系统发育分析，7 个 L-tnfr 基因分为三个不同的簇，tnfr 基因家族的多个成员在七鳃鳗中出现。同时，蛋白结构域和基序分析表明 TNFRSF 是保守的，具有典型的半胱氨酸丰富结构域（CRDs）。基因同线性结果表明，与有颚脊椎动物相比，L-tnfr 邻近基因发生了很大的变化。实时定量结果表明，tnfr 基因家族在免疫防御中发挥着重要作用。本研究为不同脊椎动物中 tnfr 基因家族的起源和进化提供了新的认识。
